Treponeme outer cell envelope: solubilization and reaggregation.

Infection and immunity ·Vol. 7 ·No. 2 ·1973-02-00 ·Pages 249-58

Johnson RC, Wachter MS, Ritzi DM

Abstract

Exposure of the avirulent Kazan 5 treponeme to low concentrations of sodium dodecyl sulfate (1.4 mm) results in the solubilization of the outer envelope. Maximal reaggregation of the outer envelope preparation requires cations, and cation concentration affects both the yield and structure of the aggregates. The reaggregated outer envelope preparation can be resolubilized with the chelating agent, ethylenediaminetetraacetic acid, or with sodium dodecyl sulfate.

MeSH Terms

Bacteriological Techniques Cell Wall/drug effects Dialysis Glycols/pharmacology Magnesium/pharmacology Microscopy, Electron Sodium Dodecyl Sulfate/pharmacology Solubility Treponema/cytology

Chemicals

Glycols Sodium Dodecyl Sulfate Magnesium

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Johnson R C

Wachter M S

Ritzi D M

References (9)

9 references, click to expand

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.7.2.249-258.1973

Published

1973-02-00

Pages

249-58

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC422668

Subset

External Links

PubMed source DOI: 10.1128/iai.7.2.249-258.1973 PMC Full Text View journal details

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Treponeme outer cell envelope: solubilization and reaggregation.

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