Home LiteratureArticle Details
PMID: 4569698 Published · ppublish English Journal Article

Isolation of covalently closed circular deoxyribonucleic acid from bacteria which produce exocellular nuclease.

Journal of bacteriology ·Vol. 113 ·No. 1 ·1973-01-00 ·Pages 508-9

Timmis K, Winkler U

Abstract

Reproducible yields of covalently closed circular (plasmid) deoxyribonucleic acid were obtained from mutants defective for extracellular nuclease but not from the corresponding wild-type strain of Serratia marcescens

MeSH Terms
Carbon Isotopes Cell-Free System Centrifugation, Density Gradient DNA Replication DNA, Bacterial/analysis,biosynthesis,isolation & purification DNA, Circular/isolation & purification Endonucleases/biosynthesis,metabolism Extrachromosomal Inheritance Mutation Serratia marcescens/analysis,enzymology,metabolism Tritium
Chemicals
Carbon Isotopes DNA, Bacterial DNA, Circular Tritium Endonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Timmis K
Winkler U
References (5)
5 references, click to expand
  1. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
  2. An extracellular nuclease from Serratia marcescens. I. Purification and some properties of the enzyme.
    J Biol Chem. 1969 Oct 10;244(19):5213-8 PMID: 4899013
  3. An extracellular nuclease from Serratia marcescens. II. Specificity of the enzyme.
    J Biol Chem. 1969 Oct 10;244(19):5219-25 PMID: 4310088
  4. Circular DNA forms of colicinogenic factors E1, E2 and E3 from Escherichia coli.
    J Mol Biol. 1968 Sep 14;36(2):185-94 PMID: 4939624
  5. The use of exocellular nuclease-negative mutants for the study of the plasmid DNA content of Serratia marcescens.
    Hoppe Seylers Z Physiol Chem. 1972 May;353(5):762-3 PMID: 4560668
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.113.1.508-509.1973
Published
1973-01-00
Pages
508-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC251656
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.113.1.508-509.1973 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2