Regulation of macromolecular biosynthesis in a mutant of Escherichia coli defective in membrane phospholipid biosynthesis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 2 ·1973-02-00 ·Pages 385-9

Glaser M, Bayer WH, Bell RM, Vagelos PR

Abstract

Nucleic acid and protein synthesis were studied in temperature-sensitive mutants defective in phospholipid synthesis. The defect is due to a single mutation in glycerol 3-phosphate acyltransferase (EC 2.3.1.15). The results show that at the restrictive temperature not only does phospholipid synthesis cease, but DNA, RNA, and protein synthesis also cease. Active transport continues, however, indicating that the cells do not become leaky or lose their energy supply. These results suggest that phospholipid synthesis is coupled to DNA, RNA, and protein synthesis.

MeSH Terms

Acyltransferases Bacterial Proteins/biosynthesis Carbon Isotopes DNA, Bacterial/biosynthesis Escherichia coli/growth & development,metabolism Glycerophosphates/metabolism Glycosides Kinetics Leucine/metabolism Membranes Mutation Phosphates/metabolism Phospholipids/biosynthesis Phosphorus Isotopes RNA, Bacterial/biosynthesis Temperature Thymidine/metabolism Tritium Uracil/metabolism

Chemicals

Bacterial Proteins Carbon Isotopes DNA, Bacterial Glycerophosphates Glycosides Phosphates Phospholipids Phosphorus Isotopes RNA, Bacterial Tritium Uracil Acyltransferases Leucine Thymidine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Glaser M

Bayer W H

Bell R M

Vagelos P R

References (39)

39 references, click to expand

Evidence for two genes specifically involved in unsaturated fatty acid biosynthesis in Escherichia coli.
J Bacteriol. 1969 Nov;100(2):601-4 PMID: 4901354
Membrane synthesis in Bacillus subtilis. II. Integration of membrane proteins in the absence of lipid synthesis.
J Mol Biol. 1970 Apr 28;49(2):433-9 PMID: 4988528
Induction of citrate transport in Bacillus subtilis during the absence of phospholipid synthesis.
J Bacteriol. 1971 May;106(2):514-8 PMID: 4324807
Lipids of Salmonella typhimurium and Escherichia coli: structure and metabolism.
J Bacteriol. 1968 Mar;95(3):833-43 PMID: 4868364
Inhibition of methylgalactoside transport in Escherichia coli upon the cessation of unsaturated fatty acid biosynthesis.
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2125-9 PMID: 4559596
Two compounds implicated in the function of the RC gene of Escherichia coli.
Nature. 1969 Mar 1;221(5183):838-41 PMID: 4885263
The fluid mosaic model of the structure of cell membranes.
Science. 1972 Feb 18;175(4023):720-31 PMID: 4333397
Mutants of Escherichia coli with temperature-sensitive lesions in membrane phospholipid synthesis: genetic analysis of glycerol-3-phosphate acyltransferase mutants.
Mol Gen Genet. 1972;116(3):199-210 PMID: 4558645
Bacteriophage Q replicase contains the protein biosynthesis elongation factors EF Tu and EF Ts.
Proc Natl Acad Sci U S A. 1972 May;69(5):1313-7 PMID: 4624757
Effect of phospholipase C hydrolysis of membrane phospholipids on membranous enzymes.
J Biol Chem. 1972 May 10;247(9):2835-41 PMID: 4337103
Effects of oleate starvation in a fatty acid auxotroph of Escherichia coli K-12.
J Bacteriol. 1969 May;98(2):784-96 PMID: 4891268
The control of ribonucleic acid synthesis in Escherichia coli. IV. Relevance of unusual phosphorylated compounds from amino acid-starved stringent strains.
J Biol Chem. 1969 Jun 25;244(12):3133-41 PMID: 4893338
Arrangement of fatty acyl groups in phosphatidylethanolamine from a fatty acid auxotroph of Escherichia coli.
Biochemistry. 1970 Sep 1;9(18):3631-40 PMID: 4324094
Induction of Staphylococcus aureus Lactose Permease in the Absence of Glycerolipid Synthesis.
Proc Natl Acad Sci U S A. 1971 Feb;68(2):420-4 PMID: 16591907
On the nature of the control by RC gene in e. coli: amino acid-dependent control of lipid synthesis.
Biochem Biophys Res Commun. 1968 Oct 10;33(1):108-12 PMID: 4880243
The specific acylation of glycerol 3-phosphate to monoacylglycerol 3-phosphate in Escherichia coli. Evidence for a single enzyme conferring this specificity.
J Biol Chem. 1970 Dec 10;245(23):6442-8 PMID: 4922042
Induction of the lactose transport system in a lipid-synthesis-defective mutant of Escherichia coli.
J Bacteriol. 1970 Aug;103(2):410-6 PMID: 4914567
Phosphate transport in Escherichia coli.
Biochim Biophys Acta. 1971 Aug 13;241(2):494-506 PMID: 4334147
Metabolism and function of the membrane phospholipids of Escherichia coli.
Biochim Biophys Acta. 1972 Feb 14;265(1):25-60 PMID: 4552305
A possible role for RNA polymerase in the initiation of M13 DNA synthesis.
Proc Natl Acad Sci U S A. 1971 Nov;68(11):2826-9 PMID: 4941987
Purification and regulatory properties of the biosynthetic L-glycerol 3-phosphate dehydrogenase from Escherichia coli.
J Biol Chem. 1969 Jun 25;244(12):3316-23 PMID: 4389388
Membrane synthesis in Bacillus subtilis. I. Isolation and properties of strains bearing mutations in glycerol metabolism.
J Mol Biol. 1970 Apr 28;49(2):415-32 PMID: 4988527
Phospholipid metabolism in the absence of net phospholipid synthesis in a glycerol-requiring mutant of Bacillus subtilis.
J Bacteriol. 1971 Sep;107(3):790-7 PMID: 4999416
Degree of participation of exogenous thymidine in the overall deoxyribonucleic acid synthesis in Escherichia coli.
J Bacteriol. 1972 May;110(2):585-91 PMID: 4553838
The effect of phospholipid fatty acid composition in membranous enzymes in Escherichia coli.
J Biol Chem. 1972 Feb 10;247(3):652-9 PMID: 4550756
A rapid method of total lipid extraction and purification.
Can J Biochem Physiol. 1959 Aug;37(8):911-7 PMID: 13671378
Respiration and protein synthesis in Escherichia coli membrane-envelope fragments. IV. Chemical and cytological characterization and biosynthetic capabilities of fragments obtained by mild procedures.
J Cell Biol. 1972 Apr;53(1):1-23 PMID: 4335249
THE CONTROL OF DISSIMILATION OF GLYCEROL AND L-ALPHA-GLYCEROPHOSPHATE IN ESCHERICHIA COLI.
J Biol Chem. 1964 Sep;239:3106-8 PMID: 14217903
The antibacterial activity of 3-decynoyl-n-acetylcysteamine. Inhibition in vivo of beta-hydroxydecanoyl thioester dehydrase.
J Biol Chem. 1968 Jun 25;243(12):3223-8 PMID: 4968523
Growth stasis by accumulated L-alpha-glycerophosphate in Escherichia coli.
J Bacteriol. 1965 Nov;90(5):1325-9 PMID: 5321485
ACTIVE TRANSPORT OF L-ALPHA-GLYCEROPHOSPHATE IN ESCHERICHIA COLI.
J Biol Chem. 1964 Sep;239:3098-105 PMID: 14217902
A lipid requirement for induction of alkaline phosphatase, one of periplasmic enzymes, in Escherichia coli.
Biochem Biophys Res Commun. 1971 Dec 17;45(6):1506-12 PMID: 4942732
Effect of glycerol deprivation on the phospholipid metabolism of a glycerol auxotroph of Staphylococcus aureus.
J Bacteriol. 1972 Feb;109(2):668-77 PMID: 5058448
The role of energy coupling in the transport of beta-galactosides by Escherichia coli.
J Biol Chem. 1966 May 25;241(10):2200-11 PMID: 5330114
A lipid requirement for induction of lactose transport in Escherichia coli.
Proc Natl Acad Sci U S A. 1969 Jul;63(3):850-5 PMID: 4899880
Control of fatty acid synthesis in bacteria.
J Bacteriol. 1972 Apr;110(1):96-102 PMID: 4622908
Selection and characterization of an E. coli mutant defective in membrane lipid biosynthesis.
Proc Natl Acad Sci U S A. 1970 Mar;65(3):737-44 PMID: 4910856
RNA-linked nascent DNA fragments in Escherichia coli.
Proc Natl Acad Sci U S A. 1972 Jul;69(7):1863-7 PMID: 4558661
Biogenesis of E. coli membrane: evidence for randomization of lipid phase.
Nat New Biol. 1971 Dec 29;234(52):264-7 PMID: 4943839

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.2.385

Published

1973-02-00

Pages

385-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433265

Subset

External Links

PubMed source DOI: 10.1073/pnas.70.2.385 PMC Full Text View journal details

Analysis Services

Regulation of macromolecular biosynthesis in a mutant of Escherichia coli defective in membrane phospholipid biosynthesis.

Contact

WeChat Official Account