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PMID: 4566990 Published · ppublish English Journal Article

Renewal of alveolar epithelium in the rat following exposure to NO2.

The American journal of pathology ·Vol. 70 ·No. 2 ·1973-02-00 ·Pages 175-98

Evans MJ, Cabral LJ, Stephens RJ, Freeman G

Abstract

This research was undertaken to study the kinetics of Type 2 cell division and the fate of the Type 2 sister cell following exposure to NO(2). To accomplish this, male rats were exposed to NO(2). Dividing cells were labeled with (3)H-TdR and studied with autoradiographic technics in the light and electron microscopes for up to 72 hours after labeling. The kinetics of cell division were determined from a curve constructed from the percent of labeled mitotic figures. The fate of the Type 2 sister cells was determined by studying tissues at 24, 48 and 72 hours after labeling with (3)H-TdR. The results show that Type 2 cells may divide and the sister cells transform into Type 1 cells. These data support an interpretation of the mechanism for cell renewal of the alveolar epithelium in which Type 2 cells are the progenitor cells for Type 1 cells.

MeSH Terms
Animals Autoradiography Basement Membrane/cytology Cell Division/drug effects Environmental Exposure Epithelial Cells Epithelium/drug effects Histological Techniques Kinetics Male Microscopy, Electron Nitrogen Dioxide/pharmacology Pulmonary Alveoli/cytology,drug effects Rats Thymidine/metabolism Time Factors Tritium
Chemicals
Tritium Nitrogen Dioxide Thymidine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Evans M J
Cabral L J
Stephens R J
Freeman G
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24 references, click to expand
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1973-02-00
Pages
175-98
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1903972
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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