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PMID: 4566193 Published · ppublish English Journal Article

Preparation and characterization of the lateral and basal plasma membranes of the rat intestinal epithelial cell.

The Biochemical journal ·Vol. 128 ·No. 5 ·1972-08-00 ·Pages 1329-38

Douglas AP, Kerley R, Isselbacher KJ

Abstract

A technique is described for the isolation of a plasma-membrane fraction from the rat intestinal epithelial cell which is distinct from the microvillus membrane of that cell. The isolated fraction contains only about 0.2% of the sucrase activity in the original homogenate and negligible quantities of nuclear and mitochondrial membrane markers. It contains 12% of the total Na(+),K(+)-dependent adenosine triphosphatase and 7% of the alkaline phosphatase, with significant increments in specific activity of these enzymes. Multiple membrane preparations were highly reproducible with respect to the specific activities of the markers studied. The small intestine of one rat yields material containing about 1.3mg of protein. In addition an assay is described suitable for determining 5'-nucleotidase in the small intestine.

MeSH Terms
Animals Cell Fractionation Cell Membrane/analysis Centrifugation, Density Gradient Cholesterol/analysis Epithelial Cells Female Glycolipids/analysis Intestines/cytology Microscopy, Electron Microscopy, Phase-Contrast Phospholipids/analysis Rats Subcellular Fractions
Chemicals
Glycolipids Phospholipids Cholesterol
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Douglas A P
Kerley R
Isselbacher K J
References (38)
38 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1281329
Published
1972-08-00
Pages
1329-38
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1174021
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1281329 PMC Full Text View journal details
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