Temperature-sensitive mutation in regulation of ribonucleic acid synthesis in Escherichia coli.

Journal of bacteriology ·Vol. 112 ·No. 3 ·1972-12-00 ·Pages 1150-6

Kuwano M, Endo H, Yamamoto M

Abstract

An Escherichia coli mutant dependent on exogenous transfer ribonucleic acid (RNA) for bulk RNA formation at 42 C has been isolated, starting from a parental strain permeable to RNA. In the absence of added transfer RNA at the high temperature, protein synthesis stopped, and the strain formed little if any ribosomal RNA.

MeSH Terms

Bacterial Proteins/biosynthesis Carbon Isotopes Chloramphenicol/pharmacology Escherichia coli/enzymology,growth & development,isolation & purification,metabolism Galactosidases/metabolism Isotope Labeling Kinetics Leucine/metabolism Mutation Nucleic Acid Hybridization RNA, Bacterial/biosynthesis RNA, Ribosomal/biosynthesis RNA, Transfer/metabolism Ribosomes/metabolism Temperature Tritium Uracil/metabolism Uridine/metabolism

Chemicals

Bacterial Proteins Carbon Isotopes RNA, Bacterial RNA, Ribosomal Tritium Uracil Chloramphenicol RNA, Transfer Galactosidases Leucine Uridine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Kuwano M

Endo H

Yamamoto M

References (14)

14 references, click to expand

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Roles of amino acid activating enzymes in cellular physiology.
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Photochemical modification of transfer RNA and its effect on aminoacyl RNA synthesis.
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Amino acid control over RNA synthesis: a re-evaluation.
Proc Natl Acad Sci U S A. 1968 Aug;60(4):1345-52 PMID: 4877268
Ribosomal protein synthesis during and after amino acid starvation in relaxed and stringent bacteria.
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Biosynthetic precursors of 30S and 50S ribosomal particles in Escherichia coli.
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Titration of the gene sites on DNA by DNA-RNA hybridization. I. Problem of measurement.
J Mol Biol. 1968 May 28;34(1):71-84 PMID: 4938544
Decreased affinity of mutant tRNA for ribosomes.
Biochem Biophys Res Commun. 1972 Mar 10;46(5):1788-93 PMID: 4552457
A temperature-sensitive mutation in Escherichia coli transfer RNA.
J Mol Biol. 1972 Aug 28;69(3):387-96 PMID: 4562708
PROTEIN AND NUCLEIC ACID SYNTHESIS IN TWO MUTANTS OF ESCHERICHIA COLI WITH TEMPERATURE-SENSITIVE AMINOACYL RIBONUCLEIC ACID SYNTHETASES.
J Bacteriol. 1965 Mar;89:706-11 PMID: 14273649
THE SPECIFIC INHIBITION OF THE ENZYMATIC AMINOACYLATION OF VALYL- AND TYROSYL-SRNA BY PERIODATE-OXIDIZED SRNA.
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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.112.3.1150-1156.1972

Published

1972-12-00

Pages

1150-6

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC251543

Subset

External Links

PubMed source DOI: 10.1128/jb.112.3.1150-1156.1972 PMC Full Text View journal details

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Temperature-sensitive mutation in regulation of ribonucleic acid synthesis in Escherichia coli.

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