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PMID: 4564585 Published · ppublish English Journal Article

Ribonucleotides covalently linked to deoxyribonucleic acid in T4 bacteriophage.

Journal of virology ·Vol. 10 ·No. 5 ·1972-11-00 ·Pages 902-8

Speyer JF, Chao J, Chao L

Abstract

Bacteriophage T4 was grown in the presence of labeled uridine. The deoxyribonucleic acid (DNA) of the phage was shown to contain covalently attached ribonucleotides. The label appears not to be internal in the DNA strands. Presumably, it is at the ends of the DNA strands and this may be related to DNA initiation.

MeSH Terms
Carbon Isotopes Centrifugation, Density Gradient Chromatography, Thin Layer Coliphages/analysis,growth & development,isolation & purification,metabolism DNA Replication DNA, Viral/analysis,biosynthesis Deoxyribonucleases Escherichia coli Hot Temperature Nucleic Acid Denaturation Pronase RNA, Viral/analysis Ribonucleases Ribonucleotides/analysis Thymidine/metabolism Tritium Uridine/metabolism
Chemicals
Carbon Isotopes DNA, Viral RNA, Viral Ribonucleotides Tritium Deoxyribonucleases Ribonucleases Pronase Thymidine Uridine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Speyer J F
Chao J
Chao L
References (38)
38 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.10.5.902-908.1972
Published
1972-11-00
Pages
902-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC356557
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.10.5.902-908.1972 PMC Full Text View journal details
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