Purification of Escherichia coli alkaline phosphatase. Improved growth conditions for the bacteria, modified methods of preparation of the enzyme.

Absorption Alkaline Phosphatase/isolation & purification Amino Acids/analysis Bacterial Proteins/analysis Chromatography, DEAE-Cellulose Chromatography, Gel Electrophoresis, Disc Enzyme Activation Escherichia coli/enzymology,growth & development Methods Time Factors Ultracentrifugation Zinc/analysis

Amino Acids Bacterial Proteins Alkaline Phosphatase Zinc