In vivo production of an RNA-DNA copolymer after infection of Escherichia coli by bacteriophage T4.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 11 ·1972-11-00 ·Pages 3165-9

Buckley PJ, Kosturko LD, Kozinski AW

Abstract

An RNA-DNA copolymer was isolated from Escherichia coli infected with bacteriophage T4. The RNA and DNA are covalently linked, and in the same polynucleotide strand. The DNA of the copolymer hybridizes specifically to the left strand of phage T4 DNA. The copolymer is produced in cells infected with amber mutants of phage T4 deficient in DNA replication and is not inhibited by the addition of chloramphenicol.

MeSH Terms

Bromodeoxyuridine/metabolism Centrifugation, Density Gradient Chloramphenicol/pharmacology Coliphages/drug effects,metabolism DNA Replication DNA, Viral/biosynthesis,isolation & purification,metabolism Deoxyribonucleases/metabolism Escherichia coli/drug effects,metabolism Hydrolysis Lysogeny Mutation Nucleic Acid Hybridization Phosphorus Isotopes RNA, Viral/biosynthesis,isolation & purification,metabolism Ribonucleases/metabolism Thymidine/metabolism Tritium

Chemicals

DNA, Viral Phosphorus Isotopes RNA, Viral Tritium Chloramphenicol Deoxyribonucleases Ribonucleases Bromodeoxyuridine Thymidine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Buckley P J

Kosturko L D

Kozinski A W

References (7)

7 references, click to expand

Early intracellular events in the replication of T4 phage DNA. I. Complex formation of replicative DNA.
Proc Natl Acad Sci U S A. 1965 Jul;54(1):273-8 PMID: 5323019
A membrane-filter technique for the detection of complementary DNA.
Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 PMID: 5963888
Fractionation of the complementary strands of coliphage T4 DNA based on the asymmetric distribution of the poly U and poly U,G binding sites.
Virology. 1968 Apr;34(4):608-16 PMID: 4870391
Unbiased participation of T4 phage DNA strands in replication.
Biochem Biophys Res Commun. 1969 Apr 29;35(2):294-9 PMID: 4890822
Molecular recombination in T4 bacteriophage deoxyribonucleic acid. I. Tertiary structure of early replicative and recombining deoxyribonucleic acid.
J Virol. 1967 Aug;1(4):758-70 PMID: 5621474
Controls and polarity of transcription during bacteriophage T4 development.
J Mol Biol. 1971 Jul 28;59(2):329-49 PMID: 5566419
A possible role for RNA polymerase in the initiation of M13 DNA synthesis.
Proc Natl Acad Sci U S A. 1971 Nov;68(11):2826-9 PMID: 4941987

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.69.11.3165

Published

1972-11-00

Pages

3165-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389727

Subset

External Links

PubMed source DOI: 10.1073/pnas.69.11.3165 PMC Full Text View journal details

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In vivo production of an RNA-DNA copolymer after infection of Escherichia coli by bacteriophage T4.

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