Genetic and physiological studies of bacteriophage T5. 3. Patterns of deoxyribonucleic acid synthesis induced by mutants of T5 and the identification of genes influencing the appearance of phage-induced dihydrofolate reductase and deoxyribonuclease.

Journal of virology ·Vol. 9 ·No. 6 ·1972-06-00 ·Pages 981-9

Hendrickson HE, McCorquodale DJ

Abstract

Patterns of deoxyribonucleic acid (DNA) metabolism in nonpermissive cells infected with amber mutants representing 29 genes of T5 are reported. A group of 7 contiguous genes are essential for the synthesis of phage DNA, whereas 20 other genes, when defective, permit varying degrees of phage DNA synthesis. Two further genes are essential for complete transfer of phage DNA to host cells, and therefore indirectly do not permit the synthesis of phage DNA. The structural genes for an early T5 deoxyribonuclease and for T5 DNA polymerase, as well as a gene that affects the synthesis of dihydrofolate reductase, have been identified in the genetic map of T5.

MeSH Terms

Carbon Isotopes Cell-Free System Coliphages/enzymology,metabolism DNA Nucleotidyltransferases/biosynthesis,metabolism DNA Replication DNA, Bacterial/metabolism DNA, Viral/biosynthesis Deoxyribonucleases/biosynthesis,metabolism Enzyme Induction Escherichia coli/metabolism Genes Genetic Complementation Test Genetics, Microbial Mutation Recombination, Genetic Spectrophotometry Tetrahydrofolate Dehydrogenase/biosynthesis,metabolism Thymidine/metabolism

Chemicals

Carbon Isotopes DNA, Bacterial DNA, Viral Tetrahydrofolate Dehydrogenase DNA Nucleotidyltransferases Deoxyribonucleases Thymidine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Hendrickson H E

McCorquodale D J

References (27)

27 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.9.6.981-989.1972

Published

1972-06-00

Pages

981-9

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC356404

Subset

External Links

PubMed source DOI: 10.1128/JVI.9.6.981-989.1972 PMC Full Text View journal details

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Genetic and physiological studies of bacteriophage T5. 3. Patterns of deoxyribonucleic acid synthesis induced by mutants of T5 and the identification of genes influencing the appearance of phage-induced dihydrofolate reductase and deoxyribonuclease.

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