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PMID: 4553840 Published · ppublish English Journal Article

Dissociation of Lac messenger ribonucleic acid transcription from translation during recovery from inhibition of protein synthesis.

Journal of bacteriology ·Vol. 110 ·No. 2 ·1972-05-00 ·Pages 652-60

Artman M, Ennis HL

Abstract

Studies were made of the synthesis of Lac messenger ribonucleic acid (mRNA) by Escherichia coli in the absence of protein synthesis and of the coupling of transcription of lac operon to translation. Lac mRNA was not synthesized in the presence of chloramphenicol, and its synthesis steadily decreased during K(+) deprivation and treatment with puromycin. Since under these conditions total mRNA synthesis is not inhibited it is suggested that the control of Lac mRNA is distinct from that which regulates total mRNA synthesis. Lac mRNA synthesized during recovery from K(+) starvation or from chloramphenicol inhibition is not translated into functional enzyme, suggesting translational control over beta-galactosidase synthesis.

MeSH Terms
Bacterial Proteins/antagonists & inhibitors,biosynthesis Carbon Isotopes Chloramphenicol/pharmacology Coliphages Culture Media Enzyme Induction Escherichia coli/drug effects,enzymology,growth & development,metabolism Galactosidases/biosynthesis Genetic Code Genetics, Microbial Leucine/metabolism Methionine/metabolism Mutation Nucleic Acid Hybridization Operon Potassium/metabolism Puromycin/pharmacology RNA, Messenger/biosynthesis Tritium Uracil/metabolism
Chemicals
Bacterial Proteins Carbon Isotopes Culture Media RNA, Messenger Tritium Puromycin Uracil Chloramphenicol Methionine Galactosidases Leucine Potassium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Artman M
Ennis H L
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22 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.110.2.652-660.1972
Published
1972-05-00
Pages
652-60
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC247461
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.110.2.652-660.1972 PMC Full Text View journal details
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