Abstract
Nitrosomonas europaea, Nitrobacter agilis, Thiobacillus denitrificans, T. neapolitanus, and T. thioparus (all obligate autotrophic bacteria) have been grown in dialysis culture, on glucose salts media, in the absence of their specific inorganic energy source. Metabolic products for N. agilis grown on nitrite salts medium were identified as keto acids. Pyruvic acid inhibited this organism at 5 x 10(-5)m. Keto acids were not inhibitory for the thiobacilli grown on thiosulfate medium. However, when T. denitrificans was grown on glucose with dialysis, addition of 5 x 10(-4)m pyruvate inhibited growth. Thus, it appears pyruvate may be inhibitory for other autotrophs, as has been reported for T. thiooxidans.
MeSH Terms
Ammonia/metabolism
Bacteriological Techniques/instrumentation
Culture Media
Dialysis
Glucose/metabolism
Keto Acids/metabolism
Methods
Nitrites/metabolism
Nitrobacter/drug effects,growth & development,metabolism
Nitrosomonas/growth & development,metabolism
Protein Biosynthesis
Pyruvates/pharmacology
Species Specificity
Thiobacillus/growth & development,metabolism
Thiosulfates/metabolism
Chemicals
Culture Media
Keto Acids
Nitrites
Pyruvates
Thiosulfates
Ammonia
Glucose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pan P
Umbreit W W
References (20)
20 references, click to expand
-
Growth response of Nitrosomonas europaea to amino acids.
J Bacteriol. 1967 Apr;93(4):1302-8
PMID: 4226683
-
MOLYBDENUM AS A MICRONUTRIENT FOR NITROBACTER.
J Bacteriol. 1965 Jan;89:123-8
PMID: 14255651
-
Reduced nicotinamide adenine dinucleotide oxidase and alpha-ketoglutaric dehydrogenase activity by Thiobacillus thiooxidans.
J Bacteriol. 1969 Feb;97(2):966-7
PMID: 4304224
-
Enzymes of carbohydrate metabolism in Thiobacillus species.
J Bacteriol. 1971 Jul;107(1):179-86
PMID: 5563867
-
Biochemical basis of obligate autotrophy in blue-green algae and thiobacilli.
J Bacteriol. 1967 Oct;94(4):972-83
PMID: 4963789
-
Characterization of ribulose diphosphate carboxylase and phosphoribulokinase from Thiobacillus thioparus and Thiobacillus neapolitanus.
Arch Biochem Biophys. 1968 Sep 20;127(1):310-6
PMID: 5697988
-
Absorption and utilization of organic matter by the strict autotroph, Thiobacillus thiooxidans, with special reference to aspartic acid.
J Bacteriol. 1966 Feb;91(2):661-6
PMID: 5934496
-
Growth of Ferrobacillus ferrooxidans on organic matter.
J Bacteriol. 1969 Jan;97(1):256-60
PMID: 5764332
-
Growth of Thiobacillus thiooxidans on glucose.
Arch Biochem Biophys. 1966 Sep 26;116(1):97-102
PMID: 5961860
-
Reduced nicotinamide adenine dinucleotide oxidation by Thiobacillus neapolitanus and Thiobacillus strain C.
J Bacteriol. 1968 May;95(5):1962-3
PMID: 4297023
-
Effect of mixed culture on Nitrosomonas europaea simulated by uptake and utilization of pyruvate.
J Bacteriol. 1966 Jan;91(1):367-73
PMID: 5903103
-
The thiobacilli.
Bacteriol Rev. 1957 Sep;21(3):195-213
PMID: 13471458
-
Acetate assimilation by Nitrobacter agilis in relation to its "obligate autotrophy".
J Bacteriol. 1968 Mar;95(3):844-55
PMID: 5643062
-
Growth of Nitrosomonas europaea in batch and in continuous culture.
Arch Mikrobiol. 1968 May 8;61(3):302-9
PMID: 4884200
-
Basis of pyruvate inhibition in Thiobacillus thiooxidans.
J Bacteriol. 1970 May;102(2):462-6
PMID: 5419262
-
Utilization of glucose and the effect of organic compounds on the chemolithotroph Thiobacillus ferrooxidans.
J Bacteriol. 1971 Oct;108(1):328-33
PMID: 5122808
-
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75
PMID: 14907713
-
Isolation of Nitrosomonas in pure culture.
J Bacteriol. 1958 Nov;76(5):524-8
PMID: 13598713
-
Stimulation of Nitrobacter agilis by Biotin.
J Bacteriol. 1965 Sep;90(3):729-33
PMID: 16562074
-
Oxidative phosphorylation in extracts of thiobacillus X.
Biochem Z. 1965 Aug 6;342(3):272-87
PMID: 4286344