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PMID: 4551747 Published · ppublish English Journal Article

Growth of obligate autotrophic bacteria on glucose in a continuous flow-through apparatus.

Journal of bacteriology ·Vol. 109 ·No. 3 ·1972-03-00 ·Pages 1149-55

Pan P, Umbreit WW

Abstract

Nitrosomonas europaea, Nitrobacter agilis, Thiobacillus denitrificans, T. neapolitanus, and T. thioparus (all obligate autotrophic bacteria) have been grown in dialysis culture, on glucose salts media, in the absence of their specific inorganic energy source. Metabolic products for N. agilis grown on nitrite salts medium were identified as keto acids. Pyruvic acid inhibited this organism at 5 x 10(-5)m. Keto acids were not inhibitory for the thiobacilli grown on thiosulfate medium. However, when T. denitrificans was grown on glucose with dialysis, addition of 5 x 10(-4)m pyruvate inhibited growth. Thus, it appears pyruvate may be inhibitory for other autotrophs, as has been reported for T. thiooxidans.

MeSH Terms
Ammonia/metabolism Bacteriological Techniques/instrumentation Culture Media Dialysis Glucose/metabolism Keto Acids/metabolism Methods Nitrites/metabolism Nitrobacter/drug effects,growth & development,metabolism Nitrosomonas/growth & development,metabolism Protein Biosynthesis Pyruvates/pharmacology Species Specificity Thiobacillus/growth & development,metabolism Thiosulfates/metabolism
Chemicals
Culture Media Keto Acids Nitrites Pyruvates Thiosulfates Ammonia Glucose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pan P
Umbreit W W
References (20)
20 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
Published
1972-03-00
Pages
1149-55
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC247337
Subset
IM
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