Cell surface galactosyltransferase and lectin agglutination of thymus and spleen lymphocytes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 9 ·1974-09-00 ·Pages 3726-30

Lamont JT, Perrotto JL, Weiser MM, Isselbacher KJ

Abstract

Lectin agglutination and cell surface galactosyltransferase (EC 2.4.1.67; 1-O-alpha-D-galactosyl-myo-inositol:raffinose galactosyltransferase) enzyme activity have been studied with thymus and spleen lymphocytes of neonatal rats. Thymus lymphocytes were more agglutinable by concanavalin A than by wheat germ agglutinin, whereas spleen lymphocytes were more agglutinable by wheat germ agglutinin than by concanavalin A. Thymus lymphocytes, but not spleen lymphocytes, of neonatal rats could be blast transformed by concanavalin A. Cell surface galactosyltransferase activity was present on both types of lymphocytes, but was greatly increased on thymus cells after blast transformation. The differences in lectin agglutination suggest a difference in the surface membranes of thymus and spleen lymphocytes. The increase in cell surface galactosyltransferase activity with blast transformation of thymus lymphocytes may be related to the exteriorization of the Golgi apparatus into the plasma membrane.

MeSH Terms

Agglutination Animals Autoradiography Cell Membrane/enzymology Concanavalin A/pharmacology Female Hexosyltransferases/metabolism Lectins/pharmacology Lymphocyte Activation Lymphocytes/enzymology Rats Spleen/cytology T-Lymphocytes/enzymology Tritium

Chemicals

Lectins Tritium Concanavalin A Hexosyltransferases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Lamont J T

Perrotto J L

Weiser M M

Isselbacher K J

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20 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.9.3726

Published

1974-09-00

Pages

3726-30

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433849

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.9.3726 PMC Full Text View journal details

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Cell surface galactosyltransferase and lectin agglutination of thymus and spleen lymphocytes.

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