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PMID: 4547208 Published · ppublish English Journal Article

Failure of lymphocytes to reexpress antigen receptors after brief interaction with a tolerogenic D-amino acid copolymer.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 8 ·1974-08-00 ·Pages 3111-4

Ault KA, Unanue ER, Katz DH, Benacerraf B

Abstract

We present data concerning a strongly tolerogenic molecule made of a copolymer of D-amino acids that is not metabolizable poly(D-glutamic acid-D-lysine). The dinitrophenyl conjugate of this molecule induces profound tolerance in dinitrophenyl-specific B lymphocytes both in vivo and in vitro. We show that dinitrophenyl-poly(D-glutamic acid-D-lysine) binds to a population of lymphocytes including those specific for dinitrophenyl, that it persists for long periods on these lymphocytes and is poorly internalized by them, and that these lymphocytes are prevented from binding other dinitrophenyl compounds. Despite prolonged culture, they do not reexpress receptors for dinitrophenyl. This behavior of dinitrophenyl-poly(D-glutamic acid-D-lysine) is contrasted to that of metabolizable antigens. This finding indicates that one mechanism of tolerance induced by nonmetabolizable antigens involves the prolonged loss of B lymphocyte receptor function.

MeSH Terms
Animals Antigens B-Lymphocytes/immunology Binding Sites, Antibody Cells, Cultured Dinitrophenols Glutamates/immunology Immune Tolerance Iodine Radioisotopes Lysine/immunology Mice Mice, Inbred A Peptides/immunology Spleen
Chemicals
Antigens Dinitrophenols Glutamates Iodine Radioisotopes Peptides Lysine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ault K A
Unanue E R
Katz D H
Benacerraf B
References (16)
16 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.8.3111
Published
1974-08-00
Pages
3111-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388631
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.8.3111 PMC Full Text View journal details
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