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PMID: 454602 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A rapid method for the isolation of kidney brush border membranes.

Biochimica et biophysica acta ·Vol. 554 ·No. 1 ·1979-06-13 ·Pages 259-63

Malathi P, Preiser H, Fairclough P, Mallett P, Crane RK

Abstract

A simple rapid method for the preparation of purified brush border membranes from rabbit kidney proximal tubules is described. The method is based on hypotonic lysis, Ca2+ aggregation of contaminants and differential centrifugation. In contrast to most other published methods, the brush border membranes are free of contamination by basolateral membranes.

MeSH Terms

Alanine/metabolism Animals Cell Fractionation/methods Cell Membrane/ultrastructure Dithiothreitol/pharmacology Glucose/metabolism Kidney Cortex/ultrastructure Kidney Tubules, Proximal/metabolism,ultrastructure Kinetics Microvilli/metabolism,ultrastructure Rabbits

Chemicals

Glucose Alanine Dithiothreitol

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Malathi P

Preiser H

Fairclough P

Mallett P

Crane R K

Article Info

Journal

Biochimica et biophysica acta

Abbr.

Biochim Biophys Acta

ISSN

0006-3002

DOI

10.1016/0005-2736(79)90023-3

Published

1979-06-13

Pages

259-63

Language

English

Region

Netherlands

NLM ID

0217513

Subset

External Links

PubMed source DOI: 10.1016/0005-2736(79)90023-3

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A rapid method for the isolation of kidney brush border membranes.

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