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PMID: 4545897 Published · ppublish English Journal Article

Separable populations of activated thymus-derived lymphocytes identified in two assays for cell-mediated immunity to murine tumor allografts.

The Journal of experimental medicine ·Vol. 140 ·No. 1 ·1974-07-01 ·Pages 267-89

Tigelaar RE, Gorczynski RM

Abstract

The immune response of C57BL mice to a DBA/2 tumor allograft has been assessed in two assays of cell-mediated immunity, the in vitro lysis of (51)Cr-labeled target cells and the antigen-mediated inhibition of macrophage migration. Both assays were shown to be measuring a T-cell-mediated reaction. Three types of experiments suggested that distinct subpopulations of T cells mediate these reactions. The tissue distributions of these activities was distinctive; both activities were present in spleens from i.p. immunized mice, but only macrophage migration inhibition activity was found in the peripheral lymph nodes (PLN) of such mice. Adoptive transfer of immune spleen cells into irradiated syngeneic recipients revealed that while a substantial amount of migration inhibition activity could subsequently be found in PLN, cytotoxic activity was found predominantly in the spleens of these adoptive hosts. Velocity sedimentation analysis of immune cells 14 days after i.p. immunization indicated that while the majority of cytotoxic activity was associated with small and medium lymphocytes, the majority of migration inhibition activity was associated with medium and large lymphocytes. In addition, normal spleen cells were fractionated by velocity sedimentation immediately before allosensitization in vitro. Subsequent analysis of the sensitized fractions revealed that the activity profiles for cytotoxicity and macrophage migration inhibition were not coincident. The implications of these observations are discussed.

MeSH Terms
Animals Antigen-Antibody Reactions Cytotoxicity Tests, Immunologic Immunity, Cellular Immunosuppression Therapy Lymph Nodes/immunology Macrophages/immunology Male Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Inbred DBA Neoplasm Transplantation Neoplasms, Experimental/immunology Spleen/immunology T-Lymphocytes/immunology
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tigelaar R E
Gorczynski R M
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.140.1.267
Published
1974-07-01
Pages
267-89
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2139711
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.140.1.267 PMC Full Text View journal details
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