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PMID: 4544056 Published · ppublish English Journal Article

Alteration in DNA synthetic response of thymocytes from NZB mice of different ages.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 12 ·1973-12-00 ·Pages 3769-72

Dauphinee MJ, Talal N

Abstract

Thymocytes from NZB and DBA/2 mice were injected into lethally irradiated C57 B1/6 recipients. DNA synthesis in spleen and lymph node was measured as the incorporation of radioactive 5-iodo-2-deoxyuridine. The generation of cytotoxic effector cells was also studied in an in vitro cytotoxicity assay, with EL-4 lymphoma target cells. The kinetics of DNA synthesis were similar for 2- and 8-week-old DBA/2 donors and for 2-week-old NZB donors with a peak on day 4. Thymocytes from 8-week-old and 9-month-old NZB donors showed a delayed onset of DNA synthesis which was still increasing on day 6. When NZB donor thymocytes from 2- and 8-week-old mice were mixed together before injections, as few as 25% of 8-week-old cells gave a DNA synthetic response characteristic of this aged population. These results suggest an abnormal thymocyte development in NZB mice which may relate to the subsequent emergence of autoimmune disease.

MeSH Terms
Age Factors Animals Cell Line Chromium Radioisotopes Cytotoxicity Tests, Immunologic DNA/biosynthesis Histocompatibility Idoxuridine/metabolism Iodine Radioisotopes Lymphocyte Activation Lymphoma/immunology Mice Mice, Inbred C57BL Mice, Inbred NZB/immunology Radiation Chimera T-Lymphocytes/immunology,metabolism,transplantation Transplantation, Homologous
Chemicals
Chromium Radioisotopes Iodine Radioisotopes DNA Idoxuridine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dauphinee M J
Talal N
References (29)
29 references, click to expand
  1. Quantitative assay of the lytic action of immune lymphoid cells on 51-Cr-labelled allogeneic target cells in vitro; inhibition by isoantibody and by drugs.
    Immunology. 1968 Feb;14(2):181-96 PMID: 4966657
  2. Deficient immunologic functions of NZB mice.
    Proc Soc Exp Biol Med. 1968 Apr;127(4):1204-7 PMID: 5655665
  3. Strain differences in the immune response of mice. I. The neonatal response to sheep red cells.
    Immunology. 1968 Jul;15(1):35-50 PMID: 5664400
  4. Autoimmune and immunoproliferative diseases of NZB/Bl mice and hybrids.
    Int Rev Exp Pathol. 1966;5:217-52 PMID: 4877346
  5. Immune response to a soluble protein antigen in NZB mice.
    Nature. 1968 Sep 21;219(5160):1276-7 PMID: 5677432
  6. Relative inability to induce tolerance in adult NZB and NZB-NZW F1 mice.
    J Exp Med. 1969 Jan 1;129(1):123-39 PMID: 4178111
  7. The immunology and pathology of NZB mice.
    Adv Immunol. 1968;9:215-66 PMID: 4883742
  8. Comparison of the immune responsiveness of NZB and NZB X NZW F1 hybrid mice with that of other strains of mice.
    J Exp Med. 1969 Nov 1;130(5):1093-105 PMID: 4186796
  9. Response of NZB and NZB-NZW spleen cells to mitogenic agents.
    J Immunol. 1970 Apr;104(4):918-23 PMID: 5442841
  10. Induction of immunologic tolerance in older New Zealand mice repopulated with young spleen, bone marrow, or thymus.
    J Exp Med. 1970 Jun 1;131(6):1223-38 PMID: 4192570
  11. Synergy among lymphoid cells mediating the graft-versus-host response. I. Synergy in graft-versus-host reactions produced by cells from NZB-Bl mice.
    J Exp Med. 1970 Feb;131(2):223-34 PMID: 4392945
  12. Depletion of long-lived lymphocytes in old New Zealand black mice.
    Clin Exp Immunol. 1970 Apr;6(4):457-72 PMID: 4920597
  13. Immunologic and viral factors in the pathogenesis of systemic lupus erythematosus.
    Arthritis Rheum. 1970 Nov-Dec;13(6):887-94 PMID: 5495398
  14. Kinetic differences in unresponsiveness of thymus and bone marrow cells.
    Science. 1971 Feb 26;171(3973):813-5 PMID: 5099924
  15. Changes in lymphoid populations of ageing CBA and NZB mice.
    Clin Exp Immunol. 1971 Mar;8(3):491-500 PMID: 5576663
  16. The age related responses of New Zealand mice to a murine sarcoma virus.
    Clin Exp Immunol. 1971 Mar;8(3):501-9 PMID: 5576664
  17. Natural thymocytotoxic autoantibody and reactive antigen in New Zealand black and other mice.
    Proc Natl Acad Sci U S A. 1971 Jul;68(7):1412-5 PMID: 5283928
  18. Inability of the NZB-NZW F 1 thymus to transfer cyclophosphamide-induced tolerance to sheep erythrocytes.
    J Immunol. 1971 Aug;107(2):359-64 PMID: 4936286
  19. Progressive loss in vitro of cellular immunity with ageing in strains of mice susceptible to autoimmune disease.
    Clin Exp Immunol. 1971 Sep;9(3):305-11 PMID: 5571055
  20. Strain differences in the immune responses of mice. 3. A raised tolerance threshold in NZB thymus cells.
    Immunology. 1971 Dec;21(6):1037-43 PMID: 4943144
  21. DNA synthetic response of thymocytes to a variety of antigens.
    J Immunol. 1972 Mar;108(3):581-5 PMID: 4401005
  22. Synergy among lymphoid cells mediating the graft-versus-host response. 3. Evidence for interaction between two types of thymus-derived cells.
    J Exp Med. 1972 Apr 1;135(4):764-79 PMID: 4401604
  23. The response of T cells to histocompatibility-2 antigens. Dose-response kinetics.
    J Exp Med. 1972 Jul 1;136(1):112-27 PMID: 4402392
  24. T and B lymphocytes in New Zealand black mice. An analysis of the theta, TL and MBLA markers.
    Clin Exp Immunol. 1972 May;11(1):1-11 PMID: 4402642
  25. Lymphocyte subpopulations in NZB mice: deficit of thymus-dependent lymphocytes.
    J Immunol. 1972 Sep;109(3):602-11 PMID: 4538300
  26. Lymphocyte classes in New Zealand mice. I. Ontogeny and mitogen responsiveness of thymocytes and thymus-derived lymphocytes.
    J Immunol. 1972 Oct;109(4):692-700 PMID: 4116356
  27. A cellular basis for genetically controlled immunologic unresponsiveness in mice: tolerance induction in T-cells.
    Proc Natl Acad Sci U S A. 1973 Jan;70(1):250-4 PMID: 4539650
  28. Age-decrease of cells sensitive to an autoantibody-specific for thymocytes and thymus-dependent lymphocytes in NZB mice.
    Clin Exp Immunol. 1972 Dec;12(4):455-64 PMID: 4539827
  29. REDUCTION OF PSEUDOMONAS AERUGINOSA AND COLIFORM BACTERIA IN MOUSE DRINKING WATER FOLLOWING TREATMENT WITH HYDROCHLORIC ACID OR CHLORINE.
    Lab Anim Care. 1963 Oct;13:737-44 PMID: 14069871
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.70.12.3769
Published
1973-12-00
Pages
3769-72
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC427324
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.70.12.3769 PMC Full Text View journal details
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