Antibodies to a specific surface antigen of T cells in human sera inhibiting mixed leukocyte culture reactions.

The Journal of experimental medicine ·Vol. 138 ·No. 4 ·1973-10-01 ·Pages 1021-6

Wernet P, Kunkel HG

Abstract

Surface radioiodination of lymphocytes by the lactoperoxidase procedure has permitted demonstration of an assortment of different antibodies to lymphocytes in the sera of patients with systemic lupus erythematosus. One type of antibody proved of special interest because it appeared to be associated with inhibition of the responder cells in the mixed leukocyte culture reactions. This reacted with an antigen on T cells and thymocytes which on sodium dodecyl sulfate acrylamide gel electrophoresis showed a molecular weight of approximately 15,000 daltons. The possible relation of this antigen to T cell receptors and products of immune response genes is discussed.

MeSH Terms

Animals Antibodies/isolation & purification Antibody Formation Antibody Specificity Antigen-Antibody Reactions Antigens Cell Membrane/immunology Electrophoresis, Polyacrylamide Gel Genes Histocompatibility Testing Humans Iodine Radioisotopes Lupus Erythematosus, Systemic/immunology Lymphocyte Activation Rabbits/immunology T-Lymphocytes/immunology

Chemicals

Antibodies Antigens Iodine Radioisotopes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Wernet P

Kunkel H G

References (11)

11 references, click to expand

Isolation of heavy and light chains of immunoglobulin from the surfaces of lymphoid cells.
J Immunol. 1971 Apr;106(4):1133-5 PMID: 5553716
Histocompatibility matching. VI. Miniaturization of the mixed leukocyte culture test: a preliminary report.
Transplantation. 1971 Mar;11(3):268-73 PMID: 5558562
Transplantation antigens: introductory symposium. Mixed leukocyte cultures and HL-A antigens. I. Reactivity of young fetuses, newborns and mothers at delivery.
Transplant Proc. 1971 Mar;3(1):58-63 PMID: 4106331
Three closely linked genetic systems relevant to transplantation.
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3031-5 PMID: 4399834
Histocompatibility-linked immune response genes.
Science. 1972 Jan 21;175(4019):273-9 PMID: 4109878
Inhibition of the mixed lymphocyte reaction by antibodies.
Transplant Proc. 1972 Jun;4(2):173-6 PMID: 4556062
Serologically defined and lymphocyte-defined components of the major histocompatibility complex in the mouse.
J Exp Med. 1972 Dec 1;136(6):1430-44 PMID: 4404878
Depressed cell-mediated immunity in patients with primary intracranial tumors. Characterization of a humoral immunosuppressive factor.
J Exp Med. 1972 Dec 1;136(6):1631-47 PMID: 4345108
Graft survival in unrelated donor--recipient pairs matched for MLC and HL-A.
Transplant Proc. 1973 Mar;5(1):409-14 PMID: 4266668
Relative T-cell specificity of lymphocytotoxins from patients with systemic lupus erythematosus.
Arthritis Rheum. 1973 May-Jun;16(3):369-75 PMID: 4575171
Serological identification of an Ir-region product.
Science. 1973 Jul 13;181(4095):167-9 PMID: 4541239

Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.138.4.1021

Published

1973-10-01

Pages

1021-6

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2180576

Subset

External Links

PubMed source DOI: 10.1084/jem.138.4.1021 PMC Full Text View journal details

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Antibodies to a specific surface antigen of T cells in human sera inhibiting mixed leukocyte culture reactions.

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