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PMID: 4537032 Published · ppublish English Journal Article

17 O nuclear magnetic resonance spectrum of H 2 17 O in frog striated muscle.

Biophysical journal ·Vol. 12 ·No. 4 ·1972-04-00 ·Pages 404-13

Civan MM, Shporer M

Abstract

Whole striated muscles from the frog Rana esculenta were bathed in Ringer's solution enriched with H(2) (17)O; the muscle water was subsequently collected by vacuum distillation. The integrated intensity of the nuclear magnetic resonance (NMR) signal of (17)O in the muscle was measured to be approximately (3/4) of the signal observed in the distilled water. The phenomenon may arise either from immobilization of a population of the water molecules which may be a very small fraction or as much as (1/4) of the total, or may reflect tumbling of (1/3) of the water molecules in a compartment containing an anisotropic medium. Such an effect was demonstrated for H(2) (17)O using the model system of sodium linoleate in water.

MeSH Terms
Animals Anura Linoleic Acids Magnetic Resonance Spectroscopy Muscles Oxygen Isotopes Rana esculenta Sodium Water
Chemicals
Linoleic Acids Oxygen Isotopes Water Sodium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Civan M M
Shporer M
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22 references, click to expand
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(72)86092-2
Published
1972-04-00
Pages
404-13
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1484123
Subset
IM
External Links
PubMed source DOI: 10.1016/S0006-3495(72)86092-2 PMC Full Text View journal details
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