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PMID: 4536669 Published · ppublish English Journal Article

Homogeneous antibody elicited with dinitrophenyl-gramicidin-S (rabbit-isoelectric focusing).

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 1 ·1972-01-00 ·Pages 228-32

Montgomery PC, Rockey JH, Williamson AR

Abstract

Rabbit antibody to dinitrophenyl hapten has been elicited by hyperimmunization with a bifunctional cyclic decapeptide antigen, di-dinitrophenyl-gramicidin-S. Examination of the induced antibody by isoelectric focusing, and Sipsian analysis of the haptenbinding data, demonstrated that di-dinitrophenyl-gramicidin-S was capable of stimulating and maintaining the production of a biosynthetically homogeneous antibody in the adult animal.

MeSH Terms
Animals Antibodies, Anti-Idiotypic Antibody Formation Antibody Specificity Antigen-Antibody Reactions Haptens Immunization Iodine Isotopes Isoelectric Focusing Nitrophenols/pharmacology Protein Binding Rabbits Tyrothricin/pharmacology
Chemicals
Antibodies, Anti-Idiotypic Haptens Iodine Isotopes Nitrophenols Tyrothricin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Montgomery P C
Rockey J H
Williamson A R
References (20)
20 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.1.228
Published
1972-01-00
Pages
228-32
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC427581
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.1.228 PMC Full Text View journal details
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