Use of eukaryotic native small ribosomal subunits for the translation of globin messenger RNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 9 ·1974-09-00 ·Pages 3435-9

Freienstein C, Blobel G

Abstract

A highly active in vitro system for the translation of globin mRNA, resulting in more than 10 rounds of translation, is described. The reconstituted system consists of native small ribosomal subunits of rabbit reticulocytes (as a source of initiation factors as well as small ribosomal subunits), large subunits derived from rat liver polysomes by the puromycin-KCl procedure, and a pH 5 fraction obtained from a Krebs ascites cell high speed supernatant. In this system no differences were found between globin messenger ribonucleoprotein and globin mRNA.

MeSH Terms

Animals Carcinoma, Krebs 2 Cell Fractionation Electrophoresis, Polyacrylamide Gel Globins/biosynthesis Hydrogen-Ion Concentration Liver/ultrastructure Mice Nucleoproteins Peptide Chain Initiation, Translational Peptide Initiation Factors Polyribosomes/analysis Potassium Chloride Protein Biosynthesis Puromycin RNA, Messenger/metabolism Rabbits Reticulocytes/metabolism,ultrastructure Ribosomes/metabolism Sodium Dodecyl Sulfate

Chemicals

Nucleoproteins Peptide Initiation Factors RNA, Messenger Sodium Dodecyl Sulfate Puromycin Potassium Chloride Globins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Freienstein C

Blobel G

References (17)

17 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.9.3435

Published

1974-09-00

Pages

3435-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433788

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.9.3435 PMC Full Text View journal details

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Use of eukaryotic native small ribosomal subunits for the translation of globin messenger RNA.

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