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PMID: 4530276 Published · ppublish English Journal Article

A limited number of globin genes in human DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 10 ·1974-10-00 ·Pages 3966-70

Gambino R, Kacian D, O'Donnell J, Ramirez F, Marks PA, Bank A

Abstract

The number of globin genes in human cells was determined by hybridizing DNA from human spleens to (3)H-labeled DNA complementary to human globin mRNA. Assuming the rates of reannealing of complementary DNA and cellular DNA are similar, the extent of hybridization of complementary DNA at various ratios of cellular DNA to complementary DNA indicate that there are fewer than 10 globin gene copies per haploid human genome. An alternative analysis of the data, which introduces no assumptions concerning the relative rates of reaction of complementary DNA and cellular DNA, indicates fewer than 20 globin gene copies are present. DNA isolated from the spleen of a patient with beta(+) thalassemia contained a number of globin gene copies similar to that of normal DNA.

MeSH Terms
Base Sequence Centrifugation, Density Gradient Chromatography DNA/analysis Genes Globins/biosynthesis Haploidy Humans Hydroxyapatites Kinetics Nucleic Acid Hybridization RNA, Messenger/isolation & purification Spleen/analysis Thalassemia/metabolism
Chemicals
Hydroxyapatites RNA, Messenger Globins DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Gambino R
Kacian D
O'Donnell J
Ramirez F
Marks P A
Bank A
References (22)
22 references, click to expand
  1. Isolation of deoxyribonucleic acid from mammalian tissues.
    Biochem J. 1967 Jul;104(1):254-7 PMID: 4291998
  2. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms.
    Science. 1968 Aug 9;161(3841):529-40 PMID: 4874239
  3. Evidence for multiple structural genes for the gamma chain of human fetal hemoglobin.
    Proc Natl Acad Sci U S A. 1968 Jun;60(2):537-44 PMID: 5248810
  4. Silent hemoglobin alpha genes in apes: potential source of thalassemia.
    Science. 1971 Jan 15;171(3967):182-5 PMID: 5540329
  5. DNA-RNA hybridization in vast DNA excess.
    Nat New Biol. 1971 May 5;231(18):8-12 PMID: 4932893
  6. Isolation and translation of hemoglobin messenger RNA from thalassemia, sickle cell anemia, and normal human reticulocytes.
    J Clin Invest. 1971 Nov;50(11):2458-60 PMID: 5096528
  7. Defect in messenger RNA for human hemoglobin synthesis in beta thalassemia.
    J Clin Invest. 1971 Dec;50(12):2755-60 PMID: 5129324
  8. In vitro synthesis of DNA complementary to purified rabbit globin mRNA (RNA-dependent DNA polymerase-reticulocyte-hemoglobin-density gradient centrifugation-oligo(dT) primer).
    Proc Natl Acad Sci U S A. 1972 Jan;69(1):264-8 PMID: 4333043
  9. In vitro synthesis of DNA complementary to rabbit reticulocyte 10S RNA.
    Nat New Biol. 1972 Feb 9;235(58):163-7 PMID: 4334193
  10. In vitro synthesis of DNA components of human genes for globins.
    Nat New Biol. 1972 Feb 9;235(58):167-9 PMID: 4334194
  11. Increased efficiency of exogenous messenger RNA translation in a Krebs ascites cell lysate.
    Proc Natl Acad Sci U S A. 1972 May;69(5):1299-303 PMID: 4338590
  12. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  13. Temperature dependence of RNA-DNA hybridization kinetics.
    Biochim Biophys Acta. 1972 Aug 16;277(1):87-95 PMID: 4559806
  14. Evidence for single copies of globin genes in the mouse genome.
    Nature. 1972 Sep 22;239(5369):219-21 PMID: 4117010
  15. A comparison of globin genes in duck reticulocytes and liver cells.
    Biochem Biophys Res Commun. 1972 Nov 1;49(3):813-9 PMID: 4638756
  16. Globin messenger-RNA induction during erythroid differentiation of cultured leukemia cells.
    Proc Natl Acad Sci U S A. 1972 Dec;69(12):3620-3 PMID: 4509323
  17. Reiteration frequency of duck haemoglobin genes.
    Nat New Biol. 1973 Feb 14;241(111):204-7 PMID: 4512585
  18. Globin synthesis of intact cells and activity of isolated mRNA in -thalassaemia.
    Nat New Biol. 1973 May 23;243(125):114-6 PMID: 4513553
  19. Quantitative deficiency of chain-specific globin messenger ribonucleic acids in the thalassemia syndromes.
    Proc Natl Acad Sci U S A. 1973 Jun;70(6):1809-13 PMID: 4124305
  20. Decreased globin messenger RNA in thalassemia detected by molecular hybridization.
    Proc Natl Acad Sci U S A. 1973 Jun;70(6):1886-90 PMID: 4124307
  21. Use of micrococcal nuclease to monitor hybridization reactions with DNA.
    Anal Biochem. 1974 Apr;58(2):534-40 PMID: 4597219
  22. Conditions for using DNA polymerase I as an RNA-dependent DNA polymerase.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1035-9 PMID: 4133845
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.10.3966
Published
1974-10-00
Pages
3966-70
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC434307
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.10.3966 PMC Full Text View journal details
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