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PMID: 4530270 Published · ppublish English Journal Article

The molecular weight of the major glycoprotein from the human erythrocyte membrane.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 10 ·1974-10-00 ·Pages 3913-6

Grefrath SP, Reynolds JA

Abstract

The molecular weight of the major glycoprotein from the human erythrocyte membrane is 29,000, of which 55% is carbohydrate and 45% is protein. The binding of sodium dodecyl sulfate to this glycoprotein is anomalous when compared to water soluble proteins and leads to migration rates in sodium dodecyl sulfate-polyacrylamide gels that cannot be interpreted in terms of molecular weight. Anomalous sodium dodecyl sulfate binding may be a general characteristic of many intrinsic membrane proteins even if they are not glycoproteins, and such proteins are likely to have mobilities in sodium dodecyl sulfate-gel electrophoresis that do not correspond to the mobilities of water soluble proteins of identical molecular weight.

MeSH Terms
Amino Acids/analysis Cell Membrane/analysis Erythrocytes/ultrastructure Fucose/analysis Glycoproteins/analysis,metabolism Hexosamines/analysis Hexoses/analysis Humans Molecular Weight Peptides/analysis Sialic Acids/analysis Sodium Dodecyl Sulfate
Chemicals
Amino Acids Glycoproteins Hexosamines Hexoses Peptides Sialic Acids Fucose Sodium Dodecyl Sulfate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Grefrath S P
Reynolds J A
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23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.10.3913
Published
1974-10-00
Pages
3913-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC434296
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.10.3913 PMC Full Text View journal details
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