Control of the initiation of DNA synthesis in 3T3 cells: serum factors.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 7 ·1974-07-00 ·Pages 2908-11

Hollwy RW, Kiernan JA

Abstract

The initiation of DNA synthesis in 3T3 cells can be controlled by a variety of different factors. Cells that are quiescent because of limited serum initiate DNA synthesis in response to the fibroblast growth factor of Gospodarowicz, to insulin, and to dexamethasone, as well as to other, unidentified biological materials. Interactions are seen between the effects of different factors.

MeSH Terms

Animals Autoradiography Cell Line Cell Nucleus/metabolism Chromatography DNA/biosynthesis Dexamethasone/pharmacology Fibroblasts/metabolism Growth Substances/blood,pharmacology Insulin/pharmacology Mice Mice, Inbred Strains/embryology Thymidine/metabolism Tritium

Chemicals

Growth Substances Insulin Tritium Dexamethasone DNA Thymidine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Hollwy R W

Kiernan J A

References (11)

11 references, click to expand

"Contact inhibition" of cell division in 3T3 cells.
Proc Natl Acad Sci U S A. 1968 May;60(1):300-4 PMID: 5241531
Buffer combinations for mammalian cell culture.
Science. 1971 Oct 29;174(4008):500-3 PMID: 5110427
Study of the growth response of normal and SV40-transformed 3T3 mouse fibroblasts with serum fractions obtained by use of organic solvents.
Exp Cell Res. 1972 Jul;73(1):262-6 PMID: 4338682
A partially purified polypeptide fraction from rat liver cell conditioned medium with multiplication-stimulating activity for embryo fibroblasts.
J Cell Physiol. 1973 Apr;81(2):153-60 PMID: 4735141
Stimulation of division of density inhibited fibroblasts by glucocorticoids.
Nature. 1973 Apr 6;242(5397):399-401 PMID: 4349600
Pituitary extracts and steroid hormones in the control of 3T3 cell growth.
Proc Natl Acad Sci U S A. 1973 Sep;70(9):2702-6 PMID: 4354860
Induction of 3T3 cell division at the monolayer stage. Early changes in macromolecular processes.
Exp Cell Res. 1973 Aug;80(2):405-14 PMID: 4355477
Amino acid requirements of the Novikoff hepatoma in vitro.
Proc Soc Exp Biol Med. 1959 Jan;100(1):115-8 PMID: 13634054
Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines.
J Cell Biol. 1963 May;17:299-313 PMID: 13985244
Steps in the neoplastic transformation of hamster embryo cells by polyoma virus.
Proc Natl Acad Sci U S A. 1963 Feb 15;49:171-9 PMID: 13997682
CLONAL GROWTH OF MAMMALIAN CELLS IN A CHEMICALLY DEFINED, SYNTHETIC MEDIUM.
Proc Natl Acad Sci U S A. 1965 Feb;53:288-93 PMID: 14294058

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.7.2908

Published

1974-07-00

Pages

2908-11

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC388582

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.7.2908 PMC Full Text View journal details

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Control of the initiation of DNA synthesis in 3T3 cells: serum factors.

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