Blastic transformtion of mouse spleen lymphocytes by a water-soluble mitogen extracted from Nocardia.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 5 ·1974-05-00 ·Pages 1602-6

Bona C, Damais C, Chedid L

Abstract

A water-soluble extract of Nocardia markedly increased in vitro [(3)H]thymidine incorporation by mouse spleen lymphocytes. The blastogenic activity of the extract and lipopolysaccharide was studied comparatively on various mouse lymphocyte subpopulations. The data obtained by [(3)H]thymidine incorporation and by electron microscopy have demonstrated that this preparation stimulates selectively mouse bone-marrow-derived cortisone-sensitive lymphocytes. This stimulation is related neither to a natural infection of mice with Nocardia organisms nor to the presence in Nocardia water-soluble mitogen of a lipopolysaccharide contaminant or of a lipopolysaccharide-like material.

MeSH Terms

Animals Cells, Cultured Cortisone/drug effects Dose-Response Relationship, Drug Drug Resistance Lectins/pharmacology Lipopolysaccharides/pharmacology,toxicity Lymphocyte Activation Lymphocytes/drug effects,metabolism Mice Mice, Inbred AKR Mice, Inbred Strains Microscopy, Electron Mitogens/isolation & purification,pharmacology,toxicity Nocardia/analysis Solutions Spleen Thymidine/metabolism Thymus Gland Tritium Tuberculin/pharmacology Water

Chemicals

Lectins Lipopolysaccharides Mitogens Solutions Tuberculin Water Tritium Cortisone Thymidine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Bona C

Damais C

Chedid L

References (16)

16 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.5.1602

Published

1974-05-00

Pages

1602-6

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC388284

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.5.1602 PMC Full Text View journal details

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Blastic transformtion of mouse spleen lymphocytes by a water-soluble mitogen extracted from Nocardia.

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