Detection and isolation of a new DNA polymerase from human breast tumor cell line HBT-3 by (dT)12-18-cellulose chromatography.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 8 ·1973-08-00 ·Pages 2453-6

Gerwin BI, Bassin RH

Abstract

Column chromatography on (dT)(12-18)-cellulose was used to detect and isolate a novel DNA polymerase activity from human breast tumor cell line HBT-3. The new enzyme is similar to those of mammalian RNA tumor viruses in its molecular size, preference for poly(A).(dT)(12-18) as compared to either poly(dA).(dT)(12-18) or poly(A).poly(dT), and ability to copy poly(C).(dG)(12-18). A similar DNA polymerase activity, defined by its binding to (dT)(12-18)-cellulose, is not detectable in several other cell lines of human tumors.

MeSH Terms

Adenine Nucleotides Breast Neoplasms/enzymology Cell Line Cellulose Centrifugation, Density Gradient Chromatography Chromatography, Affinity Chromatography, Ion Exchange DNA Nucleotidyltransferases/analysis,isolation & purification,metabolism Female Humans Polynucleotides Rauscher Virus/enzymology Templates, Genetic Thymidine Thymine Nucleotides

Chemicals

Adenine Nucleotides Polynucleotides Thymine Nucleotides Cellulose DNA Nucleotidyltransferases Thymidine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Gerwin B I

Bassin R H

References (27)

27 references, click to expand

Synthetic RNA-dependent DNA polymerase activity in normal rat liver and hepatomas.
Nature. 1972 Jun 30;237(5357):499-503 PMID: 12635194
Virus-like particles in established murine cell lines: electron-microscopic observations.
Science. 1967 Mar 24;155(3769):1543-5 PMID: 4289822
RNA-dependent DNA polymerase in virions of RNA tumour viruses.
Nature. 1970 Jun 27;226(5252):1209-11 PMID: 4316300
RNA-dependent DNA polymerase in virions of Rous sarcoma virus.
Nature. 1970 Jun 27;226(5252):1211-3 PMID: 4316301
Separation of murine cellular and murine leukaemia virus DNA polymerases.
Nat New Biol. 1971 Jun 9;231(23):163-7 PMID: 5281193
DNA polymerases from human cells.
Nat New Biol. 1971 Jun 9;231(23):167-70 PMID: 5281194
Deoxyribonucleic acid polymerase with rat liver ribosomes and smooth membranes. Purification and properties of the enzymes.
Biochemistry. 1971 May 25;10(11):1981-92 PMID: 4327392
Low molecular weight deoxyribonucleic acid polymerase in mammalian cells.
J Biol Chem. 1971 Sep 25;246(18):5835-7 PMID: 5096096
Murine leukemia virus: high-frequency activation in vitro by 5-iododeoxyuridine and 5-bromodeoxyuridine.
Science. 1971 Oct 8;174(4005):155-6 PMID: 4330367
Simultaneous detection of reverse transcriptase and high molecular weight RNA unique to oncogenic RNA viruses.
Science. 1971 Nov 19;174(4011):840-3 PMID: 4330470
A new synthetic RNA-dependent DNA polymerase from human tissue culture cells (HeLa-fibroblast-synthetic oligonucleotides-template-purified enzymes).
Proc Natl Acad Sci U S A. 1972 Feb;69(2):452-5 PMID: 4501125
A chemical model for transcriptional initiation of DNA replication.
Biochem Biophys Res Commun. 1972 Feb 16;46(3):1354-60 PMID: 5062426
Low molecular weight deoxyribonucleic acid polymerase from rabbit bone marrow.
Biochemistry. 1972 Mar 28;11(7):1264-72 PMID: 5012978
An oligonucleotide affinity column for RNA-dependent DNA polymerase from RNA tumor viruses.
Proc Natl Acad Sci U S A. 1972 Sep;69(9):2599-603 PMID: 4506781
DNA-dependent DNA polymerases I and II from normal human-blood lymphocytes.
Proc Natl Acad Sci U S A. 1972 Oct;69(10):2879-84 PMID: 4342965
Reversible inactivation of the deoxyribonucleic acid polymerase of Rauscher leukemia virus.
J Virol. 1972 Oct;10(4):885-8 PMID: 4117352
Particles containing RNA-instructed DNA polymerase and virus-related RNA in human breast cancers.
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3133-7 PMID: 4117770
Reverse transcriptase activity of human acute leukaemic cells: purification of the enzyme, response to AMV 70S RNA, and characterization of the DNA product.
Nat New Biol. 1972 Nov 15;240(98):67-72 PMID: 4117890
Human leukaemic cells contain reverse transcriptase associated with a high molecular weight virus-related RNA.
Nat New Biol. 1972 Nov 15;240(98):72-5 PMID: 4117891
Isolation of a continuous epithelioid cell line, HBT-3, from a human breast carcinoma.
Proc Soc Exp Biol Med. 1972 Nov;141(2):673-80 PMID: 4344270
The presence of a polyriboadenylic acid-dependent DNA polymerase in eukaryotic cells.
Arch Biochem Biophys. 1972 Nov;153(1):26-33 PMID: 4119571
Terminal deoxynucleotidyl transferase in a case of childhood acute lymphoblastic leukemia.
Proc Natl Acad Sci U S A. 1973 Feb;70(2):521-5 PMID: 4346893
Remarks on virus-like particles in human breast cancer.
Nature. 1973 Mar 23;242(5395):260-2 PMID: 4121275
The utilization of synthetic ribonucleic acid templates by a new deoxyribonucleic acid polymerase from cultured murine cells.
J Biol Chem. 1973 Apr 25;248(8):2678-83 PMID: 4121390
Production of virus by embryonic cultures co-cultivated with breast tumour cells or infected with milk from breast cancer patients.
Nat New Biol. 1973 Jan 10;241(106):49-52 PMID: 4121496
DEOXYRIBONUCLEIC ACID POLYMERASE IN L CELLS. I. PROPERTIES OF THE ENZYME AND ITS ACTIVITY IN SYNCHRONIZED CELL CULTURES.
Biochim Biophys Acta. 1964 Feb 17;80:193-203 PMID: 14164204
DEOXYNUCLEOTIDE-POLYMERIZING ENZYMES OF CALF THYMUS GLAND. I. LARGE SCALE PURIFICATION OF TERMINAL AND REPLICATIVE DEOXYNUCLEOTIDYL TRANSFERASES.
J Biol Chem. 1965 Aug;240:3385-91 PMID: 14321377

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.8.2453

Published

1973-08-00

Pages

2453-6

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433755

Subset

External Links

PubMed source DOI: 10.1073/pnas.70.8.2453 PMC Full Text View journal details

Analysis Services

Detection and isolation of a new DNA polymerase from human breast tumor cell line HBT-3 by (dT)12-18-cellulose chromatography.

Contact

WeChat Official Account