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PMID: 4525162 Published · ppublish English Journal Article

Dopamine-beta-hydroxylase: a tetrameric glycoprotein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 8 ·1973-08-00 ·Pages 2253-5

Wallace EF, Krantz MJ, Lovenberg W

Abstract

Dopamine-beta-hydroxylase (EC 1.14.2.1) has been isolated as a pure protein from bovine-adrenal glands. Although the molecular weight of the native protein is well established (290,000), sodium dodecyl sulfate-gel electrophoresis under dissociating conditions gave a single band with a molecular weight of about 75,000. The enzyme contains about 4% carbohydrate, which consists of residues of mannose, glucosamine, galactose, glucose, fucose, and sialic acid. The pure enzyme also contains about 4 atoms of copper per molecule. It is concluded that dopamine-beta-hydroxylase is a tetrameric glycoprotein.

MeSH Terms
Adrenal Glands/enzymology Animals Cattle Dopamine beta-Hydroxylase/analysis,isolation & purification Electrophoresis, Polyacrylamide Gel Glycoproteins/analysis Hexoses/analysis Molecular Weight Neuraminic Acids/analysis Xylose/analysis
Chemicals
Glycoproteins Hexoses Neuraminic Acids Xylose Dopamine beta-Hydroxylase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wallace E F
Krantz M J
Lovenberg W
References (19)
19 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.70.8.2253
Published
1973-08-00
Pages
2253-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433712
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.70.8.2253 PMC Full Text View journal details
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