In vivo repair of the single-strand interruptions contained in bacteriophage T5 DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 3 ·1974-03-00 ·Pages 680-4

Herman RC, Moyer RW

Abstract

Bacteriophage T5 is known to contain several unique single-strand interruptions in only one strand of the duplex DNA. Analysis of labeled parental phage DNA from infected Escherichia coli shows that these nicks are repaired in vivo to yield intact double-stranded molecules. Sealing begins at about 6 min after infection and is independent of DNA replication. Repair may be an ordered process that starts at a unique end of the molecule.

MeSH Terms

Autoradiography Coliphages/metabolism DNA Repair DNA Replication DNA Viruses/metabolism DNA, Viral/analysis Electrophoresis Kinetics Lysogeny Phosphorus Radioisotopes

Chemicals

DNA, Viral Phosphorus Radioisotopes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Herman R C

Moyer R W

References (28)

28 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.3.680

Published

1974-03-00

Pages

680-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC388076

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.3.680 PMC Full Text View journal details

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In vivo repair of the single-strand interruptions contained in bacteriophage T5 DNA.

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