Phosphorylation of human platelet myosin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 11 ·1973-11-00 ·Pages 3115-9

Adelstein RS, Conti MA, Anderson W

Abstract

A preparation extracted from human blood platelets, which incorporates (32)P from gamma-labeled AT(32)P into one of the two light chains of platelet myosin and platelet myosin head is described. This phosphorylation, which appears to be due to an endogenous kinase, is specific for the myosin light chain in that no other protein extracted in 0.6 M KCl-15 mM Tris.HCl (pH 7.5) is phosphorylated. The phosphorylated light chain, which has been purified by gel filtration, releases the covalently bound phosphate after incubation in alkali and not after incubation in acid.

MeSH Terms

Adenosine Triphosphate/metabolism Blood Platelets/metabolism Chromatography, Gel Electrophoresis, Polyacrylamide Gel Humans Myosins/blood,isolation & purification Phosphates/blood Phosphorus Radioisotopes

Chemicals

Phosphates Phosphorus Radioisotopes Adenosine Triphosphate Myosins

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Adelstein R S

Conti M A

Anderson W

References (17)

17 references, click to expand

Isolation and characterization of myosin and two myosin fragments from human blood platelets.
Proc Natl Acad Sci U S A. 1971 Nov;68(11):2703-7 PMID: 4256552
Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
Biochemistry. 1971 Jun 22;10(13):2606-17 PMID: 4326772
Human platelet actin. Isolation and properties.
J Biol Chem. 1973 Jun 10;248(11):4083-91 PMID: 4267700
DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.
Ann N Y Acad Sci. 1964 Dec 28;121:404-27 PMID: 14240539
Actomyosin-like protein in brain.
Science. 1973 Feb 2;179(4072):441-6 PMID: 4350213
Adenyl cyclase activity in human platelets.
Biochem Biophys Res Commun. 1969 Apr 29;35(2):265-72 PMID: 4306327
Cyclic AMP and platelet function.
N Engl J Med. 1972 Feb 17;286(7):358-63 PMID: 4333462
Phosphorylation of the "37000 component" of the troponin complex (troponin-t).
Biochem J. 1973 Feb;131(2):425-8 PMID: 4722446
A phosphorylated light-chain component of myosin from skeletal muscle.
Biochem J. 1973 Sep;135(1):151-64 PMID: 4776866
Dephosphorylation of the inhibitor component of troponin by phosphorylase phosphatase.
J Biol Chem. 1972 Aug 25;247(16):5275-7 PMID: 4341542
A tropomyosin-like protein from human platelets.
J Mol Biol. 1972 Jul 21;68(2):383-7 PMID: 5069793
Phosphorylation of the inhibitor component of troponin by phosphorylase kinase.
J Biol Chem. 1972 Aug 25;247(16):5272-4 PMID: 4262569
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Isolation and characterization of myosin from cloned mouse fibroblasts.
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3693-7 PMID: 4264772
Structural studies on rabbit skeletal actin. I. Isolation and characterization of the peptides produced by cyanogen bromide cleavage.
Biochemistry. 1970 Mar 17;9(6):1355-64 PMID: 5418371
Phosphorylation of rabbit muscle troponin and actin by a 3', 5'-c-AMP-dependent protein kinase.
FEBS Lett. 1972 Oct 15;27(1):89-93 PMID: 11946814
Contractile proteins of cultured cells. I. The isolation and characterization of an actin-like protein from cultured chick embryo fibroblasts.
J Biol Chem. 1972 Jul 25;247(14):4503-9 PMID: 4261397

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.11.3115

Published

1973-11-00

Pages

3115-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC427182

Subset

External Links

PubMed source DOI: 10.1073/pnas.70.11.3115 PMC Full Text View journal details

Analysis Services

Phosphorylation of human platelet myosin.

Contact

WeChat Official Account