Correlation between the mobility of inner plasma membrane structure and agglutination by concanavalin A in two cell lines of MOPC 173 plasmocytoma cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 1 ·1974-01-00 ·Pages 114-7

Guérin C, Zachowski A, Prigent B, Paraf A, Dunia I, Diawara MA, Benedetti EL

Abstract

Both the distribution of the concanavalin A-binding sites and the rearrangement of the intramembranous particles revealed by the freeze-etching technique, have been studied by means of two variants of the same cell line issued from MOPC 173 murine plasmocytoma. One variant does not agglutinate even in presence of high lectin concentration. It has been shown that the number of binding sites and affinity are almost the same in the two variants. The clustered distribution of intramembranous particles is induced by the interaction of the concanavalin A and the cell surface only in the variant which is agglutinable. From these results it became apparent that the clustered distribution of the membrane particulate components is an acquired feature of the plasma membrane accompanying cell agglutination.

MeSH Terms

Agglutination/drug effects Animals Binding Sites Cell Line Cell Membrane/metabolism Clone Cells Concanavalin A/metabolism,pharmacology Dose-Response Relationship, Drug Freeze Etching Mice Microscopy, Electron Plasmacytoma/metabolism Tritium

Chemicals

Tritium Concanavalin A

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Guérin C

Zachowski A

Prigent B

Paraf A

Dunia I

Diawara M A

Benedetti E L

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21 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.1.114

Published

1974-01-00

Pages

114-7

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC387945

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.1.114 PMC Full Text View journal details

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Correlation between the mobility of inner plasma membrane structure and agglutination by concanavalin A in two cell lines of MOPC 173 plasmocytoma cells.

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