Microsurgically fused human somatic cell hybrids: analysis and cloning.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 12 ·1973-12-00 ·Pages 3382-6

Diacumakos EG

Abstract

Cell fusion by microsurgery permits production of specified bicellular hybrid cells with very high efficiency. Systematic analysis of the fate of bicellular hybrids reveals fundamental properties of cells in accordance with cell type. Bicellular hybrids that do not proliferate survive 1-21 days; those that do proliferate form clones 8-9 days after fusion.

MeSH Terms

Animals Cell Division Cell Fusion Cell Line Cell Nucleus Cell Survival Clone Cells Female Fetus Fibroblasts HeLa Cells Humans Hybrid Cells/cytology L Cells Lesch-Nyhan Syndrome Methods Mice Mitosis Time Factors

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Diacumakos E G

References (7)

7 references, click to expand

Microsurgical studies on human cells and cloning of HeLa cells.
Nature. 1971 Jul 2;232(5305):28-32 PMID: 4933169
Chromosome displacement in and extraction from human cells at different mitotic stages.
Nature. 1971 Jul 2;232(5305):33-6 PMID: 4933170
Induction of homokaryocyte, heterokaryocyte and hybrid formation by lysolecithin.
Exp Cell Res. 1971 Aug;67(2):427-35 PMID: 4328995
Cell fusion without viruses.
Biochem J. 1971 Oct;124(5):46P-47P PMID: 5002195
Cell disjunction ("mitosis") in somatic cell reproduction.
Int Rev Cytol. 1972;33:27-43 PMID: 4562604
Fusion of mammalian somatic cells by microsurgery.
Proc Natl Acad Sci U S A. 1972 Oct;69(10):2959-62 PMID: 4507616
"Prophasing" as a possible cause of chromosome translocation in virus-fused cells.
Nat New Biol. 1973 Jun 13;243(128):208-9 PMID: 4123654

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.12.3382

Published

1973-12-00

Pages

3382-6

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC427241

Subset

External Links

PubMed source DOI: 10.1073/pnas.70.12.3382 PMC Full Text View journal details

Analysis Services

Microsurgically fused human somatic cell hybrids: analysis and cloning.

Contact

WeChat Official Account