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PMID: 4517947 Published · ppublish English Comparative Study Journal Article

Structual comparison of ectopic and normal placental alkaline phosphatase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 10 ·1973-10-00 ·Pages 2936-40

Greene PJ, Sussman HH

Abstract

An alkaline phosphatase immunochemically similar to placental alkaline phosphatase (EC 3.1.3.1) was purified from liver metastases of a giant-cell carcinoma of the lung. Some properties of its physical and chemical structure were determined and compared to those of purified placental alkaline phosphatase. The purified tumor phosphatase and the placental phosphatase were similar with regard to the following properties: (1) NH(2)-terminal sequence, (2) peptide map, (3) subunit molecular weight, and (4) isoelectric point. The physical properties and NH(2)-terminal sequence of alkaline phosphatase isoenzyme of liver differed from the placental and the tumor enzyme. The data from the present study strongly support the hypothesis that the tumor and the placental alkaline phosphatases are products of the same gene.

MeSH Terms
Alkaline Phosphatase/analysis,isolation & purification Amino Acid Sequence Carcinoma/enzymology Chromatography, DEAE-Cellulose Cross Reactions Electrophoresis, Polyacrylamide Gel Female Humans Immunoassay Isoelectric Focusing Isoenzymes/analysis Lung Neoplasms/enzymology Male Molecular Weight Peptides/analysis Placenta/enzymology
Chemicals
Isoenzymes Peptides Alkaline Phosphatase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Greene P J
Sussman H H
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24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.70.10.2936
Published
1973-10-00
Pages
2936-40
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC427142
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.70.10.2936 PMC Full Text View journal details
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