Purification of oncornaviruses by agglutination with concanacalin A (murine leukemia virus-phytohemagglutinin-friend virus).

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 5 ·1973-05-00 ·Pages 1308-12

Stewart ML, Summers DF, Soeiro R, Fields BN, Maizel JV

Abstract

Concanavalin A (Con A) has been used to rapidly and selectively agglutinate murine and avian oncornavirions from culture medium or plasma. The agglutinated virus was concentrated rapidly and gently by low-speed centrifugation and solubilization with alpha-methyl mannoside. Infectious virus was purified 2.3 times with respect to nucleic-acid content, and more than 60% of its infectivity was recovered. Infectious particles of densities 1.18 and 1.16 g/cm(3) were found in mouse cells infected with Friend virus. Con A reacted only with particles of density 1.16 g/cm(3), indicating heterogeneity with respect to carbohydrate content or structure as well as buoyant density. Electron microscopy of virus agglutinated with Con A showed a zone of Con A-glycoprotein complexes averaging 12-15 nm in thickness.

MeSH Terms

Agglutination Animals Cells, Cultured Centrifugation, Density Gradient Concanavalin A/pharmacology Culture Media Friend murine leukemia virus/analysis,growth & development,isolation & purification,pathogenicity Glycoproteins/analysis Isotope Labeling Lectins/pharmacology Methods Mice Mice, Inbred Strains Microscopy, Electron Sucrose Tritium Uridine/metabolism Viral Plaque Assay

Chemicals

Culture Media Glycoproteins Lectins Tritium Concanavalin A Sucrose Uridine

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Stewart M L

Summers D F

Soeiro R

Fields B N

Maizel J V

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26 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.5.1308

Published

1973-05-00

Pages

1308-12

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433485

Subset

External Links

PubMed source DOI: 10.1073/pnas.70.5.1308 PMC Full Text View journal details

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Purification of oncornaviruses by agglutination with concanacalin A (murine leukemia virus-phytohemagglutinin-friend virus).

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