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PMID: 4508325 Published · ppublish English Journal Article

Translational control of hemoglobin synthesis by an initiation factor required for recycling of ribosomes and for their binding to messenger RNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 11 ·1972-11-00 ·Pages 3317-21

Kaempfer R, Kaufman J

Abstract

The continued recycling of ribosomes during protein synthesis in rabbit reticulocyte lysates at 37 degrees requires an initiation factor whose activity is rapidly lost in the absence of added heme. Partially purified factor (i) fully maintains the polysomes; (ii) inhibits the association of 40S and 60S ribosomal subunits into single ribosomes; (iii) promotes the quantitative entry of added 60S subunits into polysomes; (iv) allows the accumulation of ribosomal subunits, instead of single ribosomes, when initiation is blocked with aurin tricarboxylate; and (v) is absolutely required for the binding of globin messenger RNA to ribosomes.These properties suggest that this mammalian initiation factor functions analogously to bacterial IF-3. In addition, the translational control of globin synthesis by heme is exerted, directly or indirectly, through this factor.

MeSH Terms
Animals Cell-Free System Chromatography, DEAE-Cellulose Chromatography, Gel Hemoglobins/biosynthesis Peptide Initiation Factors/metabolism Phosphorus Isotopes Protein Biosynthesis RNA, Messenger/metabolism Rabbits Reticulocytes/cytology,metabolism Ribosomes/analysis,drug effects,metabolism Tricarboxylic Acids/pharmacology
Chemicals
Hemoglobins Peptide Initiation Factors Phosphorus Isotopes RNA, Messenger Tricarboxylic Acids
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kaempfer R
Kaufman J
References (22)
22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.11.3317
Published
1972-11-00
Pages
3317-21
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389762
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.11.3317 PMC Full Text View journal details
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