A replicating RNA molecule suitable for a detailed analysis of extracellular evolution and replication.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 10 ·1972-10-00 ·Pages 3038-42

Kacian DL, Mills DR, Kramer FR, Spiegelman S

Abstract

The aim of the present study is to make available a replicating molecule of known sequence. Accordingly, we sought a molecule that has the following properties: (a) replicates in vitro in a manner similar to phage Qbeta RNA; (b) produces antiparallel complementary strands that can be separated from one another; and (c) is small enough to yield its sequence with reasonable effort. We report here the isolation of a replicating RNA molecule that contains 218 nucleotides and possesses the other features desired for a definitive analysis of the replicating mechanism. Despite its small size, this molecule can mutate to previously determined phenotypes. It will, therefore, permit the precise identification of the base changes required to mutate from one phenotype to another in the course of extracellular Darwinian selection experiments.

MeSH Terms

Base Sequence Centrifugation, Density Gradient Chromatography, Gel Coliphages/analysis,enzymology,metabolism Cytosine Nucleotides/metabolism Electrophoresis, Polyacrylamide Gel Guanine Nucleotides/metabolism Nucleic Acid Conformation Nucleic Acid Denaturation Phosphorus Isotopes RNA Nucleotidyltransferases/metabolism RNA, Viral/analysis,biosynthesis,isolation & purification Ribonucleases Tritium

Chemicals

Cytosine Nucleotides Guanine Nucleotides Phosphorus Isotopes RNA, Viral Tritium RNA Nucleotidyltransferases Ribonucleases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Kacian D L

Mills D R

Kramer F R

Spiegelman S

References (25)

25 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.69.10.3038

Published

1972-10-00

Pages

3038-42

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389702

Subset

External Links

PubMed source DOI: 10.1073/pnas.69.10.3038 PMC Full Text View journal details

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A replicating RNA molecule suitable for a detailed analysis of extracellular evolution and replication.

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