DNA polymerase of chicken embryo: purification and properties.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 7 ·1972-07-00 ·Pages 1781-5

Stavrianopoulos JG, Karkas JD, Chargaff E

Abstract

The almost complete purification of the previously reported DNA polymerase of chicken embryo is described; in the final isolation step the enrichment of the enzyme, which comprises about 0.01% of the protein extractable from 10-day-old embryos, is more than 9000-fold. The enzyme is a basic protein (pI = 9.15), of molecular weight about 27,000. It exhibits no nuclease activity, requires Mn(2+) ion for activity, and may contain Zn. It is very sensitive to oxidation.

MeSH Terms

Ammonium Sulfate Animals Centrifugation, Density Gradient Chemical Precipitation Chick Embryo/enzymology Chromatography, DEAE-Cellulose Chromatography, Gel DNA Nucleotidyltransferases/antagonists & inhibitors,isolation & purification Drug Stability Electrophoresis Enzyme Activation Isoelectric Focusing Manganese Molecular Weight Oxidation-Reduction Phenanthrolines Radioisotopes Zinc

Chemicals

Phenanthrolines Radioisotopes Manganese DNA Nucleotidyltransferases Zinc Ammonium Sulfate

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Stavrianopoulos J G

Karkas J D

Chargaff E

References (8)

8 references, click to expand

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J Biol Chem. 1969 Jun 10;244(11):2996-3008 PMID: 4890762
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
Doxynucleotide-polymerizing enzymes of calf thymus gland. IV. Inhibition of terminal deoxynucleotidyl transferase by metal ligands.
Proc Natl Acad Sci U S A. 1970 Apr;65(4):1041-8 PMID: 4985880
Zinc in DNA polymerases.
Biochem Biophys Res Commun. 1971 Jul 2;44(1):37-43 PMID: 4940372
Nucleic acid polymerases of the developing chicken embryo: a DNA polymerase preferring a hybrid template.
Proc Natl Acad Sci U S A. 1971 Sep;68(9):2207-11 PMID: 4943793
A method for determining the sedimentation behavior of enzymes: application to protein mixtures.
J Biol Chem. 1961 May;236:1372-9 PMID: 13767412
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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.69.7.1781

Published

1972-07-00

Pages

1781-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC426801

Subset

External Links

PubMed source DOI: 10.1073/pnas.69.7.1781 PMC Full Text View journal details

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