Initiation of protein synthesis in HeLa cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 6 ·1972-06-00 ·Pages 1375-9

Chatterjee NK, Kerwar SS, Weissbach H

Abstract

Initiation of protein synthesis in HeLa cells has been synchronized by exposure of the cells to fluoride. Double-labeling of such cells for short pulses with [(35)S]methionine and a tritiated amino acid, followed by Edman degradation of the puromycin-released nascent peptides, has shown that the percent of N-terminal methionine incorporated compared to total incorporation is significantly higher than the value obtained with any of the other amino acids tested. The results suggest that the bulk of the nascent proteins synthesized in vivo by HeLa cells are initiated with methionine.

MeSH Terms

Amino Acids/metabolism Fluorides/pharmacology HeLa Cells/drug effects,metabolism Isotope Labeling Methionine/metabolism Peptide Biosynthesis Peptide Chain Initiation, Translational/drug effects Potassium/pharmacology Protein Biosynthesis Puromycin/pharmacology Sulfur Isotopes Time Factors Tritium

Chemicals

Amino Acids Sulfur Isotopes Tritium Puromycin Methionine Fluorides Potassium

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Chatterjee N K

Kerwar S S

Weissbach H

References (29)

29 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.69.6.1375

Published

1972-06-00

Pages

1375-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC426705

Subset

External Links

PubMed source DOI: 10.1073/pnas.69.6.1375 PMC Full Text View journal details

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