Citrate lyase: a pantothenate-containing enzyme.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 5 ·1972-05-00 ·Pages 1201-2

Srere PA, Böttger B, Brooks GC

Abstract

Citrate lyase from Klebsiella aerogenes inactivated by reaction in the presence of substrate or by treatment with hydroxylamine can be reactivated with acetic anhydride only if its sulfhydryl groups are reduced. Alkaline hydrolysis of pure citrate lyase yields about 3 mol of phosphopantothenate per mol of enzyme.

MeSH Terms

Acetates Anhydrides Chemical Phenomena Chemistry Citrates Dealkylation Dithiothreitol Enzyme Activation Hydroxylamines Klebsiella/enzymology Lyases/analysis Oxidation-Reduction Pantothenic Acid/analysis Sulfhydryl Compounds

Chemicals

Acetates Anhydrides Citrates Hydroxylamines Sulfhydryl Compounds Pantothenic Acid Lyases Dithiothreitol

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Srere P A

Böttger B

Brooks G C

References (7)

7 references, click to expand

Acyl carrier protein. VII. The primary structure of the substrate-binding site.
J Biol Chem. 1965 Dec;240(12):4723-6 PMID: 5321310
Studies on the mechanism of fatty acid synthesis. XIV. The prosthetic group of acyl carrier protein and the mode of its attachment to the protein.
J Biol Chem. 1965 Dec;240(12):4727-33 PMID: 5321311
The reaction inactivation of citrate lyase from Aerobacter aerogenes.
J Biol Chem. 1971 Jun 25;246(12):3847-50 PMID: 4327189
Attempts to map a process evolution of peptide biosynthesis.
Science. 1971 Sep 3;173(4000):875-84 PMID: 4937229
[The action mechanism of citrate lyase from Klebsiella aerogenes].
Hoppe Seylers Z Physiol Chem. 1971 Sep;352(9):1195-205 PMID: 5096065
Genetic control of repression of alkaline phosphatase in E. coli.
J Mol Biol. 1961 Aug;3:425-38 PMID: 13725581
Citritase, the citrate-splitting enzyme from Escherichia coli. II. Reaction mechanisms.
J Biol Chem. 1955 Dec;217(2):909-20 PMID: 13271451

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.69.5.1201

Published

1972-05-00

Pages

1201-2

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC426663

Subset

External Links

PubMed source DOI: 10.1073/pnas.69.5.1201 PMC Full Text View journal details

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Citrate lyase: a pantothenate-containing enzyme.

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