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PMID: 4502945 Published · ppublish English Journal Article

Genetic variants of glucose 6-phosphate dehydrogenase from human erythrocytes: unique properties of the A - variant isolated from "deficient" cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 4 ·1972-04-00 ·Pages 946-50

Babalola O, Cancedda R, Luzzatto L

Abstract

The A(-) type of glucose 6-phosphate dehydrogenase (EC 1.1.1.49) has been isolated from human erythrocytes deficient in this enzyme. The specific activity of the purified protein is similar to that previously reported for the enzyme isolated from normal, nondeficient erythrocytes. During the purification procedure, a portion of the A(-) enzyme converts spontaneously, from the native "fraction I", to a "fraction II" having different kinetic and chromatographic properties. The conversion of fraction I to II can be reproduced freely by treatment with iodosobenzoate, and fraction II can be converted back to fraction I by treatment with dithioglycol. We suggest that fraction II is an enzyme species in which one or more sulfhydryl groups have been oxidized to disulfide(s). The tendency to oxidation appears to be a property specific to the A(-) variant and may represent the basis for its rapid rate of inactivation and consequent deficiency in vivo.

MeSH Terms
Blood Protein Electrophoresis Centrifugation, Density Gradient Chromatography, Gel Disulfides/biosynthesis Electrophoresis, Starch Gel Enzyme Activation Erythrocytes/enzymology Genetic Variation Glucosephosphate Dehydrogenase/blood,isolation & purification Humans Kinetics Models, Structural Molecular Biology Oxidation-Reduction Protein Conformation Sulfhydryl Compounds/metabolism Temperature
Chemicals
Disulfides Sulfhydryl Compounds Glucosephosphate Dehydrogenase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Babalola O
Cancedda R
Luzzatto L
References (24)
24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.4.946
Published
1972-04-00
Pages
946-50
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC426601
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.4.946 PMC Full Text View journal details
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