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PMID: 444219 Published · ppublish English Journal Article

The effect of starvation on the rate of protein synthesis in rat liver and small intestine.

The Biochemical journal ·Vol. 178 ·No. 2 ·1979-02-15 ·Pages 373-9

McNurlan MA, Tomkins AM, Garlick PJ

Abstract

1. A method is described that allows for measurement of protein synthesis in liver and intestine in the rat. By injecting a massive amount of [14C]leucine (100 mumol/100 g body wt.) an attempt has been made to over come problems of precursor specific radioactivity and problems arising from the breakdown of labelled protein that are encountered when tracer amounts of amino acids are used. 2. Starvation for 2 days resulted in decline in the rate of total liver protein synthesis from 87%/day to 62%/day. 3. In jejunal mucosa the rate of protein synthesis was 136%/day. This declined to 105%/day after 2 days of starvation.

MeSH Terms
Animals Carbon Radioisotopes Intestinal Mucosa/metabolism Jejunum/metabolism Leucine/metabolism Liver/metabolism Male Protein Biosynthesis Rats Starvation/metabolism Time Factors
Chemicals
Carbon Radioisotopes Leucine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
McNurlan M A
Tomkins A M
Garlick P J
References (22)
22 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1780373
Published
1979-02-15
Pages
373-9
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1186525
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1780373 PMC Full Text View journal details
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