Autolysis of Neisseria gonorrhoeae.

Journal of bacteriology ·Vol. 126 ·No. 2 ·1976-05-00 ·Pages 969-76

Elmros T, Burman LG, Bloom GD

Abstract

Autolysis of Neisseria gonorrhoeae was studied under different conditions. It was found that low pH and temperature, as well as the presence of divalent cations, spermine, sucrose, and polyvinylpyrrolidone, stabilized nongrowing gonococci. Ethylenediaminetetraacetic acid alone promoted lysis, whereas lysozyme had only a limited additive effect. The autolytic behavior of gonococci appears to be connected with their prolonged cell division process. The relative dependence on the outer membrane and the peptidoglycan layer for the mechanical stability of gonococci is discussed.

MeSH Terms

Bacteriolysis/drug effects Buffers Cell Wall/ultrastructure Culture Media Edetic Acid/pharmacology Hydrogen-Ion Concentration Metals/pharmacology Muramidase/pharmacology Neisseria gonorrhoeae/ultrastructure Povidone/pharmacology Spermine/pharmacology Sucrose/pharmacology Temperature

Chemicals

Buffers Culture Media Metals Spermine Sucrose Edetic Acid Muramidase Povidone

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Elmros T

Burman L G

Bloom G D

References (21)

21 references, click to expand

Lipid composition and permeability of liposomes.
Biochim Biophys Acta. 1968 Jun 11;150(4):666-75 PMID: 5690752
Biosynthesis of the peptidoglycan of bacterial cell walls. XIV. Purification and properties of two D-alanine carboxypeptidases from Escherichia coli.
J Biol Chem. 1968 Jun 10;243(11):3193-201 PMID: 4871206
NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION.
J Bacteriol. 1963 Jun;85:1274-9 PMID: 14047217
Cell division in a chain-forming envA mutant of Escherichia coli K12. Fine structure of division sites and effects of EDTA, lysozyme and ampicillin.
Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;79(5):651-64 PMID: 4999789
Use of bacteriolytic enzymes in determination of wall structure and their role in cell metabolism.
Bacteriol Rev. 1968 Dec;32(4 Pt 2):425-64 PMID: 4884715
Factors affecting autolysis of Neisseria gonorrhoeae.
Proc Soc Exp Biol Med. 1974 Apr;145(4):1418-21 PMID: 4208046
Studies of the phospholipids and morphology of protoplasts of Bacillus megaterium.
Biochim Biophys Acta. 1967;135(5):862-84 PMID: 4965164
Role of Multivalent Cations in the Organization, Structure, and Assembly of the Cell Wall of Pseudomonas aeruginosa.
J Bacteriol. 1966 Aug;92(2):380-7 PMID: 16562124
Dense cultures of Neisseria gonorrhoeae in liquid medium.
Appl Microbiol. 1967 Mar;15(2):219-23 PMID: 4961767
Conditions affecting the viability of spheroplasts in urine.
Infect Immun. 1970 Mar;1(3):300-4 PMID: 16557732
Comparison of transportation media for the culturing of N. gonorrhoeae.
Am J Clin Pathol. 1974 Sep;62(3):435-7 PMID: 4213008
Micrococcus lysodeikticus bacterial walls as a substrate specific for the autolytic glycosidase of Bacillus subtilis.
J Bacteriol. 1973 May;114(2):804-13 PMID: 4196257
Autolysis of Neisseria gonorrhoeae.
J Bacteriol. 1975 May;122(2):385-92 PMID: 236277
LYSIS OF CELL WALLS AND INTACT CELLS OF PSEUDOMONAS AERUGINOSA BY ETHYLENEDIAMINE TETRAACETIC ACID AND BY LYSOZYME.
Can J Microbiol. 1965 Apr;11:193-201 PMID: 14323031
DEVELOPMENT OF L-TYPE GROWTH IN NEISSERIA GONORRHOEAE CULTURES.
J Bacteriol. 1964 Jun;87:1471-6 PMID: 14188730
Thermal lysis of bacterial membranes and its prevention by polyamines.
J Gen Microbiol. 1971 May;66(2):133-5 PMID: 4999070
Cell envelope of Neisseria gonorrhoeae: outer membrane and peptidoglycan composition of penicillin-sensitive and-resistant strains.
Infect Immun. 1975 Jun;11(6):1332-41 PMID: 806526
Effect of ethylenediaminetetraacetic acid-Tris(hydroxymethyl)aminomethane on release of the acid-soluble nucleotide pool and on breakdown of ribosomal ribonucleic acid in Escherichia coli.
J Bacteriol. 1967 Apr;93(4):1360-8 PMID: 4962058
Supramolecular structure of the rigid layer of the cell wall of Salmonella, Serratia, Proteus, and Pseudomonas fluorescens. Number of lipoprotein molecules in a membrane layer.
Biochemistry. 1970 Dec 22;9(26):5041-9 PMID: 4249403
Autolytic enzymes and cell division of Escherichia coli.
J Mol Biol. 1969 May 14;41(3):419-29 PMID: 4896021
Study of the N-acetylmuramyl-L-alanine amidase activity in Escherichia coli.
FEBS Lett. 1971 Jun 10;15(2):137-141 PMID: 11945831

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.126.2.969-976.1976

Published

1976-05-00

Pages

969-76

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC233235

Subset

External Links

PubMed source DOI: 10.1128/jb.126.2.969-976.1976 PMC Full Text View journal details

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