Preparation of immunologlobulin light-chain mRNA from microsomes without the use of detergent.

Animals Autoradiography Cells, Cultured Centrifugation, Density Gradient Cytoplasm/metabolism Electrophoresis, Polyacrylamide Gel Immunoglobulin G/biosynthesis In Vitro Techniques Membranes/metabolism Methods Mice Microsomes/metabolism Mitochondria/analysis Nucleoproteins/analysis Phosphorus Radioisotopes Plasmacytoma/metabolism Puromycin/pharmacology RNA/isolation & purification RNA, Messenger/analysis,isolation & purification,metabolism Ribonucleases Ribosomes/metabolism Ultracentrifugation

Immunoglobulin G Nucleoproteins Phosphorus Radioisotopes RNA, Messenger Puromycin RNA Ribonucleases