A general method for isolation of individual poly-ribosomes and pure mRNAs by immunosorption technique.

Molecular biology reports ·Vol. 1 ·No. 7 ·1974-09-00 ·Pages 401-8

Sidorova EV, Trudolyubova MG, Lerman MI

Abstract

暂无摘要

MeSH Terms

Animals Antigen-Antibody Reactions Cell Line Centrifugation, Density Gradient Chromatography, DEAE-Cellulose Electrophoresis, Polyacrylamide Gel Immunoglobulin G/biosynthesis Liver/analysis,metabolism Male Methods Mice Mice, Inbred BALB C Osmolar Concentration Plasmacytoma Polyribosomes/immunology,metabolism Potassium Chloride Protein Biosynthesis RNA, Messenger/immunology,isolation & purification,metabolism RNA, Neoplasm/isolation & purification,metabolism RNA, Ribosomal/immunology,isolation & purification,metabolism Rabbits/immunology Rats Serum Albumin/biosynthesis Species Specificity

Chemicals

Immunoglobulin G RNA, Messenger RNA, Neoplasm RNA, Ribosomal Serum Albumin Potassium Chloride

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Sidorova E V

Trudolyubova M G

Lerman M I

References (12)

12 references, click to expand

Purification and properties of ovalbumin messenger RNA.
Eur J Biochem. 1974 Apr 16;43(3):549-60 PMID: 4831975
Purification of ovalbumin messenger ribonucleic acid by specific immunoadsorption of ovalbumin-synthesizing polysomes and millipore partition of ribonucleic acid.
J Biol Chem. 1973 Jan 25;248(2):540-8 PMID: 4119369
Variations in the heavy polypeptide chain structure of gamma myeloma immunoglobulins from an inbred strain of mice and a hypothesis as to their origin.
J Mol Biol. 1965 Dec;14(2):361-72 PMID: 4160338
Isolation of messenger RNA coding for mouse heavy-chain immunoglobulin.
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1127-31 PMID: 4515608
Functional differences in protein synthesis between free and bound polysomes of rat liver.
Biochim Biophys Acta. 1970 Sep 17;217(1):148-58 PMID: 5505328
Precipitation of polyribosomes with pepsin digested antibodies.
Biochem Biophys Res Commun. 1971 Oct 1;45(1):240-5 PMID: 4946407
Incorporation of radioactive amino acids into serum albumin by isolated rat-liver ribosomes.
Biochem J. 1962 Apr;83:69-74 PMID: 14458456
Studies on free and membrane-bound ribosomes in rat liver. I. Distribution as related to total cellular RNA.
J Mol Biol. 1967 Jun 14;26(2):279-92 PMID: 6034775
USE OF ANTIBODIES ON AN INSOLUBLE SUPPORT FOR SPECIFIC DETECTION OF RADIOACTIVE ANTIGENS.
Nature. 1964 Aug 8;203:648-9 PMID: 14250983
Identification and isolation of ovalbumin-synthesizing polysomes. 3. Precipitation of ovalbumin polysomes from a heterologous cell-free protein-synthesizing system.
J Biol Chem. 1973 Feb 25;248(4):1424-30 PMID: 4686930
Biologically and chemically pure mRNA coding for a mouse immunoglobulin L-chain prepared with the aid of antibodies and immobilized oligothymidine.
Proc Natl Acad Sci U S A. 1973 Aug;70(8):2256-60 PMID: 4525163
Dissociation of mammalian polyribosomes into subunits by puromycin.
Proc Natl Acad Sci U S A. 1971 Feb;68(2):390-4 PMID: 5277091

Article Info

Journal

Molecular biology reports

Abbr.

Mol Biol Rep

ISSN

0301-4851

DOI

10.1007/BF00385673

Published

1974-09-00

Pages

401-8

Language

English

Region

Netherlands

NLM ID

0403234

Subset

External Links

PubMed source DOI: 10.1007/BF00385673 View journal details

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A general method for isolation of individual poly-ribosomes and pure mRNAs by immunosorption technique.

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