Levels of some enzymes acting on DNA in xeroderma pigmentosum.

Nucleic acids research ·Vol. 1 ·No. 2 ·1974-02-00 ·Pages 193-202

Pedrini AM, Dalpra L, Ciarrocchi G, Noy GC, Spadari S, Nuzzo F, Falaschi A

Abstract

We have determined the levels of DNA polymerase, DNA ligase, a DNase acting on single-stranded DNA, an endonuclease making single-strand breaks in double - stranded DNA and polynucleotide kinase in fibroblasts obtained from nine normal persons and from nine patients with Xeroderma Pigmentosum; the pathological lines belong to the different described clinical forms and to the three different complementation groups described so far. All the enzymes are present in the normal lines and in the Xeroderma lines. The levels are quite variable, but the values obtained in the pathological lines lie within the ones observed in the normal population.

MeSH Terms

Adolescent Adult Cells, Cultured Child DNA/metabolism DNA Nucleotidyltransferases/metabolism DNA Repair DNA Replication DNA, Single-Stranded Deoxyribonucleases/metabolism Endonucleases/metabolism Female Fetus Fibroblasts/enzymology Genetic Complementation Test Humans Infant Lymphocytes/enzymology Male Middle Aged Phosphotransferases/metabolism Polynucleotide Ligases/metabolism Polynucleotides Pregnancy Purine-Pyrimidine Metabolism, Inborn Errors/enzymology Xeroderma Pigmentosum/enzymology,genetics

Chemicals

DNA, Single-Stranded Polynucleotides DNA Phosphotransferases DNA Nucleotidyltransferases Deoxyribonucleases Endonucleases Polynucleotide Ligases

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Pedrini A M

Dalpra L

Ciarrocchi G

Noy G C

Spadari S

Nuzzo F

Falaschi A

References (11)

11 references, click to expand

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Defective repair replication of DNA in xeroderma pigmentosum.
Nature. 1968 May 18;218(5142):652-6 PMID: 5655953
Xeroderma pigmentosum: a human disease in which an initial stage of DNA repair is defective.
Proc Natl Acad Sci U S A. 1969 Jun;63(2):428-35 PMID: 5257133
Evidence that xeroderma pigmentosum cells do not perform the first step in the repair of ultraviolet damage to their DNA.
Proc Natl Acad Sci U S A. 1969 Nov;64(3):1035-41 PMID: 5264135
Different inherited levels of DNA repair replication in xeroderma pigmentosum cell strains after exposure to ultraviolet irradiation.
Mutat Res. 1970 May;9(5):507-16 PMID: 4193204
Purification and properties of a polynucleotide ligase from human cell cultures.
Eur J Biochem. 1971 Sep 13;22(1):75-8 PMID: 5099218
Induction of polynucleotide ligase in human lymphocytes stimulated by phytohemoagglutinin.
Biochem Biophys Res Commun. 1972 Jun 9;47(5):1221-7 PMID: 5029865
Genetic heterogeneity of xeroderma pigmentosum demonstrated by somatic cell hybridization.
Nat New Biol. 1972 Jul 19;238(81):80-3 PMID: 4505415
A UV-specific endonucleolytic activity present in human cell extracts.
Biochem Biophys Res Commun. 1972 Aug 7;48(3):662-9 PMID: 4340112
Effects of inhibitors on repair of DNA in normal human and xeroderma pigmentosum cells after exposure to x-rays and ultraviolet irradiation.
Mutat Res. 1973 Mar;17(3):385-94 PMID: 4631741
UV-induced DNA repair synthesis in cells of patients with different forms of xeroderma pigmentosum and of heterozygotes.
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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/1.2.193

Published

1974-02-00

Pages

193-202

Language

English

Region

England

NLM ID

0411011

PMCID

PMC343338

Subset

External Links

PubMed source DOI: 10.1093/nar/1.2.193 PMC Full Text View journal details

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Levels of some enzymes acting on DNA in xeroderma pigmentosum.

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