Activation of leukemia viruses by graft-versus-host and mixed lymphocyte reactions in vitro.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 5 ·1972-05-00 ·Pages 1069-72

Hirsch MS, Phillips SM, Solnik C, Black PH, Schwartz RS, Carpenter CB

Abstract

Spleen cells from BALB/c or CAF(1) mice released little or no detectable leukemia virus when cultured 2-7 days in vitro. In contrast, spleen cells of CAF(1) mice previously inoculated with parental BALB/c spleen cells released leukemia viruses in 10 of 11 cases studied. Cultures of a mixture of spleen cells from normal BALB/c and CAF(1) mice also contained leukemia viruses. Phytohemagglutinin induced the transformation of lymphocytes in cultures of CAF(1) or BALB/c spleen cells, but this transformation did not activate leukemia viruses. It is concluded that mixed lymphocyte cultures in vitro, just as graft-versus-host reactions in vivo, can activate leukemia viruses that are normally present in a repressed form. This activation is not solely a function of lymphocyte transformation. The activated mouse leukemia virus may subsequently account for the observed high incidence of neoplasia in graft-versus-host disease.

MeSH Terms

Animals Cell Transformation, Neoplastic/drug effects Cells, Cultured/immunology Graft vs Host Reaction Lectins/pharmacology Leukemia Virus, Murine/immunology Lymphocytes/immunology Male Mice Mice, Inbred Strains Mitomycins Species Specificity Spleen/immunology

Chemicals

Lectins Mitomycins

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Hirsch M S

Phillips S M

Solnik C

Black P H

Schwartz R S

Carpenter C B

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25 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.69.5.1069

Published

1972-05-00

Pages

1069-72

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC426630

Subset

External Links

PubMed source DOI: 10.1073/pnas.69.5.1069 PMC Full Text View journal details

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Activation of leukemia viruses by graft-versus-host and mixed lymphocyte reactions in vitro.

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