Mutant strains of Escherichia coli K12 that use D-amino acids.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 10 ·1971-10-00 ·Pages 2484-7

Kuhn J, Somerville RL

Abstract

A series of mutations has been isolated that confer upon amino-acid auxotrophs of Escherichia coli K-12 the ability to grow when fed various D-amino acids. Several distinct systems, mediating cellular use of the D-isomers of leucine, histidine, phenylalanine, tyrosine, tryptophan, isoleucine, and valine, can be mutationally activated. Mutations leading to D-tryptophan use (dadR) all map near purB. They result in high activities of an enzyme that deaminates D-amino acids. Neither the enzymes of the tryptophan biosynthetic pathway nor tryptophanase (EC 4.2.1.e) are involved in D-tryptophan utilization.

MeSH Terms

Amino Acids/metabolism Arginine/metabolism Chromosome Mapping Coliphages D-Amino-Acid Oxidase/analysis Escherichia coli/enzymology,metabolism Genetic Linkage Genotype Histidine/metabolism Isoleucine/metabolism Isomerism Leucine/metabolism Lysine/metabolism Mutation Phenylalanine/metabolism Proline/metabolism Serine/metabolism Threonine/metabolism Transduction, Genetic Tryptophan/metabolism Tyrosine/metabolism Valine/metabolism

Chemicals

Amino Acids Isoleucine Threonine Tyrosine Serine Phenylalanine Histidine Tryptophan Arginine Proline D-Amino-Acid Oxidase Leucine Valine Lysine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kuhn J

Somerville R L

References (15)

15 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.10.2484

Published

1971-10-00

Pages

2484-7

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389449

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.10.2484 PMC Full Text View journal details

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Mutant strains of Escherichia coli K12 that use D-amino acids.

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