Appearance of T-cell markers in bone marrow rosette-forming cells after incubation with thymosin, a thymic hormone.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 11 ·1971-11-00 ·Pages 2734-8

Bach JF, Dardenne M, Goldstein AL, Guha A, White A

Abstract

After incubation with thymosin, a thymic hormone, normal bone marrow rosette-forming cells acquire T-cell characteristics, including increased sensitivity to azathioprine, anti-lymphocyte serum, and anti-theta serum. This activity of thymosin provides a new sensitive and reproducible bioassay for thymosin, and is well correlated with an in vivo graft-versus-host assay. In addition, incubation of spleen cells from adult thymectomized mice with thymosin in vitro restores to normal their diminished sensitivity to azathioprine and anti-lymphocte serum.

MeSH Terms

Animals Antibody-Producing Cells Antilymphocyte Serum/pharmacology Azathioprine/pharmacology Biological Assay Bone Marrow/drug effects,immunology Bone Marrow Cells Brain/immunology Cattle Endotoxins Graft vs Host Reaction Hormones/analysis,isolation & purification,pharmacology Humans Immune Sera/pharmacology Mice Mice, Inbred Strains Rabbits Salmonella/immunology Sheep Spleen/immunology Swine Thymectomy Thymus Gland/cytology,metabolism

Chemicals

Antilymphocyte Serum Endotoxins Hormones Immune Sera Azathioprine

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Bach J F

Dardenne M

Goldstein A L

Guha A

White A

References (19)

19 references, click to expand

Preparation, assay, and partial purification of a thymic lymphocytopoietic factor (thymosin).
Proc Natl Acad Sci U S A. 1966 Sep;56(3):1010-7 PMID: 5230175
In vitro detection of immunosuppressive activity of anti-lymphocyte sera.
Nature. 1968 Feb 17;217(5129):658-9 PMID: 5300099
Influence of thymosin on immunological competence of lymphoid cells from thymectomized mice.
Nature. 1968 Sep 28;219(5161):1391-2 PMID: 5678029
Effect of thymosin and an antithymosin serum on allograft survival in mice.
Proc Natl Acad Sci U S A. 1968 Nov;61(3):875-82 PMID: 4879824
In vitro evaluation of immunosuppressive drugs.
Nature. 1969 Jun 7;222(5197):998-9 PMID: 5815252
Influence of thymosin on cell-mediated and humoral immune responses in normal and in immunologically deficient mice.
J Immunol. 1970 Feb;104(2):359-66 PMID: 4904881
Serologic demonstration of a thymus-dependent population of lymph-node cells.
J Immunol. 1970 Apr;104(4):798-804 PMID: 5442838
Acceleration of lymphoid tissue regeneration in x-irradiated CBA-W mice by injection of thymosin.
Radiat Res. 1970 Mar;41(3):579-93 PMID: 5442949
[Analytic study of 69 anti-mouse lymphocyte rabbit serums. I. "In vivo" activity].
Pathol Biol (Paris). 1970 May;18(9):471-8 PMID: 4912159
In vivo specific antigen recognition by rosette forming cells.
Nature. 1970 Sep 19;227(5264):1251-2 PMID: 5465666
Acceleration by thymosin of the development of resistance to murine sarcoma virus-induced tumor in mice.
Proc Natl Acad Sci U S A. 1970 Aug;66(4):1170-4 PMID: 5273447
Ontogenesis of cell-mediated immunity in murine thymocytes and spleen cells and its acceleration by thymosin, a thymic hormone.
J Immunol. 1971 Mar;106(3):773-80 PMID: 4396053
[Studies on the origin of cells forming spontaneous rosettes].
C R Acad Sci Hebd Seances Acad Sci D. 1971 Mar 1;272(9):1318-9 PMID: 4994744
Early affect of adult thymectomy.
Nat New Biol. 1971 May 26;231(21):110-1 PMID: 5281184
The thymus gland: experimental and clinical studies of its role in the development and expression of immune functions.
Adv Metab Disord. 1971;5:149-82 PMID: 4934166
Immunologic and anatomic consequences of calf thymosin injection in rats.
Cell Immunol. 1970 May;1(1):51-61 PMID: 4108780
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Graft versus host reactions. Their natural history, and applicability as tools of research.
Prog Allergy. 1962;6:349-467 PMID: 14037719
THE AKR THYMIC ANTIGEN AND ITS DISTRIBUTION IN LEUKEMIAS AND NERVOUS TISSUES.
J Exp Med. 1964 Sep 1;120:413-33 PMID: 14207060

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.11.2734

Published

1971-11-00

Pages

2734-8

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389512

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.11.2734 PMC Full Text View journal details

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Appearance of T-cell markers in bone marrow rosette-forming cells after incubation with thymosin, a thymic hormone.

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