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PMID: 4389749 Published · ppublish English Journal Article

D-amino acid oxidase in leukocytes: a possible D-amino-acid-linked antimicrobial system.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 62 ·No. 3 ·1969-03-00 ·Pages 756-63

Cline MJ, Lehrer RI

Abstract

D-Amino acid oxidase has been identified within the granule fraction of human neutrophilic leukocytes. Leukocyte homogenates and purified kidney D-amino acid oxidase can utilize either isolated D-amino acids or some species of bacteria as substrates for the generation of hydrogen peroxide. When linked to leukocyte myeloperoxidase in vitro, purified D-amino acid oxidase constitutes a system lethal for certain bacteria. It is proposed that leukocyte D-amino acid oxidase and myeloperoxidase constitute a biochemically specific system for the recognition and killing of certain microorganisms.

MeSH Terms
Animals Anti-Bacterial Agents D-Amino-Acid Oxidase/blood,metabolism Escherichia coli/drug effects Guinea Pigs Humans Hydrogen Peroxide/biosynthesis Leukocytes/enzymology Methods Neutrophils/enzymology Peroxidases/pharmacology Proteus/drug effects Serratia marcescens/drug effects Staphylococcus/drug effects
Chemicals
Anti-Bacterial Agents Hydrogen Peroxide Peroxidases D-Amino-Acid Oxidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cline M J
Lehrer R I
References (21)
21 references, click to expand
  1. Metabolism of the circulating leukocyte.
    Physiol Rev. 1965 Oct;45(4):674-720 PMID: 5318995
  2. Combined biochemical and morphological study of particulate fractions from rat liver. Analysis of preparations enriched in lysosomes or in particles containing urate oxidase, D-amino acid oxidase, and catalase.
    J Cell Biol. 1965 Jul;26(1):219-43 PMID: 4379260
  3. Cationic proteins of polymorphonuclear leukocyte lysosomes. II. Composition, properties, and mechanism of antibacterial action.
    J Bacteriol. 1966 Feb;91(2):755-62 PMID: 5934974
  4. In vitro bactericidal capacity of human polymorphonuclear leukocytes: diminished activity in chronic granulomatous disease of childhood.
    J Clin Invest. 1967 Apr;46(4):668-79 PMID: 6021213
  5. Alpha-glucosidase activity in human leucocytes.
    Biochim Biophys Acta. 1966 Jul 27;124(1):34-8 PMID: 5229655
  6. Studies of the metabolic activity of leukocytes from patients with a genetic abnormality of phagocytic function.
    J Clin Invest. 1967 Sep;46(9):1422-32 PMID: 6036538
  7. [Electron microscopic studies on peroxidase and acid phosphatase reaction in human leukocytes (in normal and leukemic cells and on phagocytosis)].
    Nihon Ketsueki Gakkai Zasshi. 1966 Aug;29(4):554-70 PMID: 5231666
  8. Role of the phagocyte in host-parasite interactions. XII. Hydrogen peroxide-myeloperoxidase bactericidal system in the phagocyte.
    J Bacteriol. 1967 Nov;94(5):1425-30 PMID: 4964478
  9. Iodination of bacteria: a bactericidal mechanism.
    J Exp Med. 1967 Dec 1;126(6):1063-78 PMID: 4964565
  10. The role of the phagocyte in host-parasite interactions. 13. The direct quantitative estimation of H2O2 in phagocytizing cells.
    Biochim Biophys Acta. 1968 Feb 1;156(1):168-78 PMID: 4868928
  11. Myeloperoxidase-halide-hydrogen peroxide antibacterial system.
    J Bacteriol. 1968 Jun;95(6):2131-8 PMID: 4970226
  12. The interaction of human monocytes and lymphocytes.
    J Exp Med. 1968 Dec 1;128(6):1309-25 PMID: 5688078
  13. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  14. The isolation and properties of the specific cytoplasmic granules of rabbit polymorphonuclear leucocytes.
    J Exp Med. 1960 Dec 1;112:983-1004 PMID: 13694490
  15. The purification and some properties of D-amino acid oxidase.
    Biochim Biophys Acta. 1961 Mar 18;48:1-9 PMID: 13767909
  16. Leukocyte preparations from human blood: evaluation of their morphologic and metabolic state.
    J Lab Clin Med. 1962 May;59:779-91 PMID: 13891473
  17. Metabolic basis of phagocytic activity.
    Physiol Rev. 1962 Jan;42:143-68 PMID: 14454025
  18. Myeloperoxidase of the leucocyte of normal human blood. I. Content and localization.
    Arch Biochem Biophys. 1962 Mar;96:465-7 PMID: 13909511
  19. MYELOPEROXIDASE OF THE LEUCOCYTE OF NORMAL HUMAN BLOOD. II. ISOLATION, SPECTROPHOTOMETRY, AND AMINO ACID ANALYSIS.
    Biochemistry. 1964 Sep;3:1234-8 PMID: 14229665
  20. INACTIVATION OF ESTROGEN BY RAT UTERINE PREPARATIONS.
    Endocrinology. 1965 Feb;76:301-11 PMID: 14262662
  21. D-AMINO ACID OXIDASE. I. DISSOCIATION AND RECOMBINATION OF THE HOLOENZYME.
    Biochim Biophys Acta. 1965 Mar 22;96:357-67 PMID: 14314378
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.62.3.756
Published
1969-03-00
Pages
756-63
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC223663
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.62.3.756 PMC Full Text View journal details
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