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PMID: 4382257 Published · ppublish English Journal Article

Changes in the enzyme pattern of the mammary gland of the lactating rat after hypophysectomy and weaning.

The Biochemical journal ·Vol. 103 ·No. 2 ·1967-05-00 ·Pages 420-7

Jones EA

Abstract

1. The enzymes glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, phosphoglucomutase, UDP-glucose pyrophosphorylase, phosphofructokinase, ATP-citrate lyase and acetyl-CoA carboxylase have been assayed in rat mammary glands in various stages of involution after hypophysectomy and weaning. 2. After hypophysectomy all seven enzymes decline in activity over a 12-16hr. period but the extent of the decline varies, with acetyl-CoA carboxylase becoming almost totally inactive, ATP-citrate lyase and phosphofructokinase showing a large decrease, and the remaining enzymes a less marked decline. 3. Within 24hr. of removing the litter a change in the pattern of enzyme activity is found very similar to that after hypophysectomy. 4. The significance of these results is discussed in relation to the endocrine control of mammary gland metabolism and the mechanisms of involution.

MeSH Terms
Animals Female Glucosephosphate Dehydrogenase/biosynthesis Hypophysectomy Kinetics Lactation/physiology Ligases/biosynthesis Lyases/biosynthesis Mammary Glands, Animal/enzymology NAD NADP Nucleotidyltransferases/biosynthesis Oxytocin/pharmacology Phosphofructokinase-1/biosynthesis Phosphoglucomutase/biosynthesis Phosphogluconate Dehydrogenase/biosynthesis Pituitary Gland/physiology Pregnancy Rats Spectrophotometry Ultracentrifugation Weaning
Chemicals
NAD Oxytocin NADP Phosphogluconate Dehydrogenase Glucosephosphate Dehydrogenase Phosphofructokinase-1 Nucleotidyltransferases Lyases Phosphoglucomutase Ligases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Jones E A
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38 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1030420
Published
1967-05-00
Pages
420-7
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1270424
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1030420 PMC Full Text View journal details
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