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PMID: 438132 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Bidirectional replication of the mini-ColE1 plasmid pVH51.

Journal of bacteriology ·Vol. 138 ·No. 1 ·1979-04-00 ·Pages 257-60

Eichenlaub R, Sparks RB, Helinski DR

Abstract

Replicating molecules of the min-ColE1 plasmid pVH51 have been examined by electron microscopy after cleavage with the restriction endonuclease EcoRI. Replication apparently starts at a unique site indistinguishable from the origin of replication used by the parental plasmid ColE1. In contrast to ColE1, the structure of the majority of the replication intermediates was consistent with a bidirectional mode of replication. A minor portion of the molecules appeared to replicate unidirectionally in either direction from the same origin.

MeSH Terms
DNA Replication DNA Restriction Enzymes DNA, Recombinant/biosynthesis Genes Plasmids
Chemicals
DNA, Recombinant DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Eichenlaub R
Sparks R B
Helinski D R
References (12)
12 references, click to expand
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  2. Bidirection replication from a unique origin in a mini-F plasmid.
    Proc Natl Acad Sci U S A. 1977 Mar;74(3):1138-41 PMID: 322144
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    Proc Natl Acad Sci U S A. 1974 Jun;71(6):2256-9 PMID: 4601583
  8. Bidirectional replication of plasmid R6K DNA in Escherichia coli; correspondence between origin of replication and position of single-strand break in relaxed complex.
    Proc Natl Acad Sci U S A. 1975 Aug;72(8):2905-9 PMID: 1103126
  9. Two replication initiation sites on R-plasmid DNA.
    Mol Gen Genet. 1975 Sep 15;140(1):39-50 PMID: 1102950
  10. Replication control in a composite plasmid constructed by in vitro linkage of two distinct replicons.
    Nature. 1976 Jan 29;259(5541):285-90 PMID: 765836
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    J Bacteriol. 1976 Apr;126(1):447-53 PMID: 770430
  12. Position of branch points in replicating lambda DNA.
    J Mol Biol. 1970 Jul 14;51(1):61-73 PMID: 5481280
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.138.1.257-260.1979
Published
1979-04-00
Pages
257-60
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC218267
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.138.1.257-260.1979 PMC Full Text View journal details
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